FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_C09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_C09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450408
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA17550.3896467203069217No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT9120.20248308200564819No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT8270.1836113035292446No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT7310.1622972948970711No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC7240.16074314843430845No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG7150.15874496012504216No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT6930.15386049981350244No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG6430.14275945365091205No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG6310.14009520257189037No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC6280.13942913980213495No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA6250.13876307703237953No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC6030.13387861672083978No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA5900.13099234471856627No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC5870.13032628194881085No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC5790.12855011456279639No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG5790.12855011456279639No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG5200.11545088009093976No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC5110.1134526917816735No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG4860.10790216870037832No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG4730.10501589669810482No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA4710.10457185485160121No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG4650.10323972931209037No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG4530.10057547823306867No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG4510.10013143638656506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA900.080.604191
TTTACGA502.305038E-672.495453
CGAAAGC1050.069.0432743
AGTAGAA4900.065.141351
GTAGAAA5800.055.0026552
AGCTATC2100.051.782469
GCGAAAG1600.049.8461532
TTATAGG1056.862501E-848.362511
CAATTCG2900.047.497029
GAGCTAT2400.045.3096548
CCCTTAT7850.045.2820971
ACAAGGC4650.045.2122158
ACTGATC2000.043.497278
TAGAAAC7350.043.3986363
CCTTATA8250.043.0626832
TTATACT8800.042.838224
GGATAGC2057.2759576E-1238.8999945
GTACTGA3000.038.6642386
GGTACTG2650.035.5638055
TAAGATG2650.035.5638055
Produced by FastQC (version 0.11.5)