FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432307
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC19520.45153097220262456No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT19390.4485238499492259No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC12760.29516061502589597No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG12670.2930787611581584No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG12020.2780431498911653No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG9960.23039182802961786No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC8620.19939533710997046No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC8340.19291845841034264No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC7940.18366577455373148No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT7690.1778828471433495No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA7530.17418177360070505No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA7460.1725625539257981No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT6970.16122801620144941No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA6790.1570643084659744No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT6600.1526692836340841No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA6320.1461924049344563No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG5480.12676176883557289No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT5360.12398596367858951No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG5160.11935962175028395No Hit
TTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAG5130.1186656704610381No Hit
TGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGA5000.11565854820763947No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT4620.1068684985438589No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA4610.10663718144744361No Hit
GTTCCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAA4400.10177952242272274No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC4390.10154820532630746No Hit
ATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACTCG4340.10039161984423106No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACCG350.003565866562.1396334
TAAGATG5400.059.071015
GCATTAA6050.052.7733731
AGATGTA6000.050.747377
GCGAAAG901.3212339E-648.3364222
TTAAGAT6200.047.9410634
CGAAAGC951.919021E-645.78713
AGCGAAA1002.7141505E-643.538031
CATTAAG7450.042.821522
ATTAAGA7550.041.289253
TTAACGT907.477667E-540.2756924
TAACGTG951.0282364E-438.155925
GCTATTG2109.094947E-1237.974228
GGACTAC800.002026625236.281691
ATTAACG1001.390567E-436.248123
AAGATGT9750.035.690466
GATTAGC3050.035.686911
GTCTAGT5900.034.436861
AACGTGT1102.4346146E-432.9528356
TATACCC2008.929419E-932.623315
Produced by FastQC (version 0.11.5)