FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_B09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_B09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872033
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA20540.23554154487272846No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT18740.21490012419254778No Hit
CCATTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA13730.15744816996604488No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG13590.15584272613536415No Hit
ATATAATGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT12970.14873290345663526No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA12850.14735680874462317No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG12270.14070568430323166No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC12160.13944426415055394No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC11570.13267846514982803No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC11130.1276327845391172No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA10940.1254539679117648No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG10650.12212840569106903No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC10600.12155503289439736No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT9920.11375716285966242No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC9610.11020225152029797No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA9390.10767941121494254No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG9140.10481254723158412No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG9030.10355112707890642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAT3950.056.896333
CAATTCG2600.055.7668769
TCGACTC400.006051639654.3727045
AGTAGAA5250.049.746411
GGTACTG2800.049.1943445
AGCGAAA1509.094947E-1248.3645671
GTCTAAG6800.048.008941
ATATAAT5050.047.4068531
CCTTATA4150.047.1666762
GTAGAAA5650.046.1927382
GGATAGC3850.043.3098565
GACAGTA4050.042.9611437
CCCTTAT4250.040.9676321
TTATACT4300.040.4634064
TCTAAGG7950.040.124092
TGATGCA13350.039.099479
ACTGATC3750.038.6650288
AGGTCGT1354.972062E-737.5910036
TTATATG6100.036.84274
GACAACG800.002036895136.2484668
Produced by FastQC (version 0.11.5)