FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_B01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_B01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409145
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG23190.16456787626539499No Hit
ATCCTATAGCGTCCGTTCTGTCTAGTTGGTGCTTGTCCCCTGCTGGTTGT14820.1051701563714167No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA14450.10254445071302101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5900.066.3804241
CGAAAGC5900.066.356873
ACCGTCA451.19415316E-464.443078
AATAGCG753.7582686E-758.009056145
TTAGTTG5950.036.555144
AGTACGT1251.2656385E-534.799268
GTACGTA1251.2656385E-534.799269
AAACGTT1051.8543325E-434.5230756
TAGCCGT850.002742650734.116926
TATAGAC9400.032.3963782
ATAGGGA4100.031.8297213
GTATTAC6500.031.2422981
ACTACTC3250.031.2312133
CGGGTAT1402.7445374E-531.07628145
TACACCG4750.028.9993825
GTCTAGA2802.6193447E-1028.4926571
CAGCTCG9950.028.4164819
TTATCCG1306.4837374E-427.885014
GTATTAA4700.027.7762091
GCGAAAG14100.027.7683242
Produced by FastQC (version 0.11.5)