FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_A12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_A12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244293
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC19940.8162329661513019No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT16590.6791025530817502No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC13700.5608019877769728No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG13180.5395160729124453No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG12590.5153647464315392No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG11830.4842545631679991No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA9920.40606976049252336No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC8590.3516269397813282No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG8480.3471241500984473No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC8150.33361578104980494No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA8140.33320643653317944No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC7940.32501954620066886No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC7770.3180606894180349No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG7670.31396724425177963No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT7140.29227198487062667No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT7000.2865411616378693No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT6690.2738514816224779No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA6520.266892624839844No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT6360.2603431125738355No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA6180.252974911274576No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA6140.2513375332080739No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG5970.24437867642543992No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT5690.2329170299599252No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA5610.22964227382692096No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG5560.22759555124379333No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG5520.2259581731772912No Hit
TTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAG5460.223502106077538No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC5390.22063669446115933No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG4900.2005788131465085No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG4880.19976012411325744No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA4710.19280126733062347No Hit
GTTCCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAA4580.18747978861449163No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT4570.18707044409786608No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT4550.18625175506461503No Hit
TGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGA4460.1825676544149853No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC4380.17929289828198106No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA4370.17888355376535553No Hit
ATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACTCG4290.1756087976323513No Hit
GTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCACCCGCATAAT4240.17356207504922366No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA4210.17233404149934708No Hit
CTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTA4120.16864994084971735No Hit
CCTAAATTCCGTATGGGAGCTTCCTGGTAACTCGTTCTGTTCCCTAATAC4050.16578452923333864No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA3980.16291911761695996No Hit
GTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAAT3830.15677894986757707No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC3610.14777337050181544No Hit
GATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAA3560.1457266479186878No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC3540.14490795888543676No Hit
GAATGGAACATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATC3540.14490795888543676No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC3480.1424518917856836No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC3430.14040516920255594No Hit
GTGTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAA3340.1367210685529262No Hit
GTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTAT3290.13467434596979858No Hit
GTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAG3270.1338556569365475No Hit
CTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGT3230.1322182788700454No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT3200.1309902453201688No Hit
TTATCAGCCATTAGCTGGTTTCCTGTGTCCCAGCAAATGCTGAGGTAAGT3110.12730614467053905No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT3040.12444073305416037No Hit
GGGAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTA3020.12362204402090933No Hit
ATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTT3000.12280335498765825No Hit
GTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAATGTGCAATA2990.12239401047103274No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2960.12116597692115616No Hit
TGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTGG2930.11993794337127957No Hit
CTTTTGGGCAGCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGG2910.11911925433802852No Hit
GTACTAGTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAGTT2820.11543515368839877No Hit
GAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAA2790.11420712013852218No Hit
GTGTACTAGTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAG2760.1129790865886456No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA2680.10970433045564137No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT2650.1084762969057648No Hit
GGTAAGTCTTGATTGCCAATATTGCACATTTCAAACATGAATCCGTAGAC2580.1056108852893861No Hit
CAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTG2570.10520154077276057No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA2530.10356416270625847No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC2520.10315481818963293No Hit
GTATGGGAGCTTCCTGGTAACTCGTTCTGTTCCCTAATACTCATGCTGTC2520.10315481818963293No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG2500.10233612915638189No Hit
GTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACT2450.10028940657325425No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA501.8189894E-12116.0665361
TTAAGAT3700.076.4314354
TAAGATG3900.074.3711555
CGAAAGC1051.6370905E-1162.1530383
AAGATGT4600.061.477466
TTAGTAC1300.061.35623
GCGAAAG1007.1122486E-1058.0332681
AGATGTA5050.057.4233937
ATTAAGA5050.055.9992753
AGTAGAA4550.054.2068941
GTAGAAA4650.053.0194362
CCCTTAT3500.049.7427981
TAGTACT2050.049.5203064
TAGAAAC5800.045.007373
CAATTCG4850.044.8435259
CGAGACA657.327134E-444.61356145
TATATTC2450.044.3859378
CCTTATA3450.044.137662
GGTCAAA2650.043.7986871
GGATAGC855.3244483E-542.6540455
Produced by FastQC (version 0.11.5)