FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_A09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_A09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297759
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG35851.2039938339395282No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA26300.8832646536292774No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC25070.8419560785736114No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20840.6998948814309559No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20370.6841103039706609No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT18610.6250020990129602No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA18420.6186210996141175No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG17880.6004856276384593No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG17080.5736182617485953No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC16880.5669014202761293No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT16460.5527960531839508No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG15240.5118233202019082No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT13980.4695072189253725No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC12050.40468969871607574No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT10280.34524565168475174No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT10220.34323059924301197No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC9150.30729549736531897No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA8620.28949586746328404No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT8600.2888241833160375No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC8570.28781665709516757No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC8110.2723679217084958No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA7970.2676661326777696No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG7860.2639718698679133No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA7590.2549041338800842No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC7570.25423244973283765No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGG7440.24986650277573474No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA7200.24180629300877554No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC7140.23979124056703574No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG6560.22031240029688437No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG6440.21628229541340477No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT6420.2156106112661582No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA6380.214267242971665No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT6370.2139314008980417No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG6320.21225219052992522No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG6220.2088937697936922No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC6080.20419198076296602No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA6040.20284861246847283No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG5910.19848266551136992No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC5740.19277335025977385No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG5650.18975077159716414No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC5430.18236224597745154No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA5380.18068303560933507No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG5330.17900382524121855No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG5160.17329450998962248No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC5110.17161529962150598No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG5070.1702719313270128No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA5050.16960024717976618No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA4960.1665776685171565No Hit
GCTCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT4890.16422677400179342No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG4880.16389093192817009No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC4830.16221172156005362No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG4570.15347982764584783No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA4510.15146477520410803No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC4480.15045724898323812No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT4290.14407624958439544No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC4250.14273288128990225No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT4060.13635188189105957No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG3940.13232177700757997No Hit
CCATGTACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGT3930.13198593493395666No Hit
GTTCATTGATGCTTAATGCTGGGCCATTGCCATCATCCACTTCATTCTGA3880.13030672456584017No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA3880.13030672456584017No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG3850.12929919834497025No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA3800.12761998797685378No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG3800.12761998797685378No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG3790.12728414590323045No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG3780.12694830382960717No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC3760.12627661968236056No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA3730.12526909346149065No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA3700.12426156724062076No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT3690.12392572516699747No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT3680.12358988309337418No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG3650.12258235687250428No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG3610.12123898857801109No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTTGAACAACTTCCAT3600.12090314650438777No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT3600.12090314650438777No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA3560.11955977820989458No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG3490.11720888369453149No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG3460.11620135747366159No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT3400.11418630503192179No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA3380.11351462088467518No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA3360.1128429367374286No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA3330.1118354105165587No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACACAG3300.1108278842956888No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA3270.1098203580748189No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA3190.1071336214858325No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA3150.1057902531913393No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC3130.10511856904409271No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA3130.10511856904409271No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG3070.10310351660235291No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT3040.10209599038148301No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA3010.10108846416061312No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA3010.10108846416061312No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG3000.10075262208698982No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT2980.10008093793974322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATG100.007078835145.065351
TCTGCCG100.0070895087144.992284
TAGTTGA100.0070895087144.992285
ACTAGTT100.0070895087144.992283
GTAGCAC100.0070895087144.992283
CTAGTTG100.0070895087144.992284
CTGCCGT100.0070895087144.992285
TTCTGCC100.0070895087144.992283
GCAGGTA1150.0126.101412
CAGGTAC1150.0126.080233
AGCGAAA950.0122.160291
GCGAAAG1050.0117.394422
CGAAAGC1050.0110.47033
GGTACTG1400.0103.565925
AGTAGAA4250.0102.399071
AGGTACT1600.090.620174
ACTGATC1650.087.87418
CCGCACA259.3697E-487.0246145
GATCTTA851.8189894E-1276.799311
GAAAGCA1600.072.496144
Produced by FastQC (version 0.11.5)