FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_A07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_A07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758818
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA10750.1416676989739305No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG9830.1295435796198825No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT9690.12769860493557084No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC9160.12071405791639102No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC7630.10055112029498509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1600.063.462621
GACAGTA3200.052.105967
CGAAAGC2000.050.7466773
ATATAAT1751.8189894E-1245.5894741
AGTAGAA5600.040.1498221
GCTATAC2005.456968E-1239.872393
GCGAAAG2550.039.8013152
TAGTACC750.001483637438.664144
TATAGTA1051.8540089E-434.521552
CAATTCG3400.034.1154149
TATTACA5750.034.041252
CCTTATA4900.034.0283852
GTATTAC5150.033.7997861
GTAGAAA7150.033.474791
CCAATAT3350.032.4605642
ATAGACA5700.031.7961653
TTATACT6850.031.7497464
GGTTCGC1601.8705377E-631.731311
ATATAGT4850.031.4041841
GTACGCT1902.0766674E-730.5243173
Produced by FastQC (version 0.11.5)