FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941091
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA27890.29635816302567974No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT22600.24014680833203167No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG22110.23494008549651413No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC19570.2079501344715867No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT19240.20444356603134023No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA17640.18744202207863No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA17140.1821290395934081No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC15520.164914976341289No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC14260.1515262604785297No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG13460.14302548850217459No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG11900.12644898314828215No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11870.12613020419916882No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC11830.12570516560035108No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG11640.12368623225596674No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC11430.12145477961217352No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG11030.11720439362399597No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC10670.1133790462346362No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC10490.11146637253995628No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC10160.1079598040997098No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT10150.10785354445000536No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG10110.1074285058511876No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG10070.10700346725236985No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT10000.1062596497044388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2300.097.752751
GCGAAAG3050.073.69562
CGAAAGC3300.068.108983
AGTAGAA8900.067.6368261
TCGTAGC350.003566582762.142224
GTAGAAA10750.056.6566052
ATTAAGA8100.055.496213
CAATTCG8650.052.7973149
TTAAGAT8950.049.4129034
TATCTCG604.934824E-448.353394145
TAAGATG9000.047.524765
GACAGTA5650.047.4748657
AGCTATC2600.047.3983279
CGGTTCG2100.044.8971941
ACTGATC5450.042.5638478
TAGAAAC14850.042.476573
GTTCGCA2300.040.980023
GGTTCGC2450.038.4730832
CAGTATG6800.038.377889
ACAATTC12350.036.9794968
Produced by FastQC (version 0.11.5)