FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_H09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_H09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018046
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT43140.4237529541887105No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC39660.38956982297460035No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC39170.384756680935832No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC26530.2605972618133169No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG25740.2528372981181597No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG23810.233879412128725No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG23150.22739640448466966No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC22270.2187523942925958No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA18390.1806401675366339No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA18330.18005080320535613No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA16910.16610251403178247No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC16910.16610251403178247No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC15200.14930563059036625No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA14880.14616235415688486No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT14750.14488539810578305No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC14030.1378130261304499No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT14020.13771479874190362No Hit
GTTAATGGGATGTCATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGG13790.13545556880533885No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT13710.1346697496969685No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC13270.13034774460093157No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT13230.1299548350467464No Hit
GTCTGGGAGAGCCTAAACGCCCGGAGCCCAGCAGGTGCTTTAACGCGAGA12130.11914982230665412No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA11910.11698881975863566No Hit
CTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTA11790.11581009109608013No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA11750.11541718154189497No Hit
GAAACAAGGAGGGGAACCAAAGGTCGAGGTGGCAGAGGGGAAATGAATGG11730.11522072676480237No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT11490.11286326943969133No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG11020.10824658217801553No Hit
TGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGA10930.1073625356810989No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG10720.10529976052162672No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA10660.10471039619034896No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT10570.10382634969343231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3450.073.579691
GCGAAAG4100.061.893332
TATACGT753.762525E-757.9971924
CGAAAGC4400.057.667663
GTAGAAA11850.053.8608631
TAAGATG14600.053.1309855
TAGCGGT604.946304E-448.3309947
CGGCTAC450.00964102948.3309947
GGGCGAT1056.909249E-848.330997
GCATTAA15850.047.132121
AGATGTA16000.046.6696137
TAGAAAC15150.042.5928762
GTCTAGT12700.042.260731
TTAAGAT17950.041.5996554
CTATACC1252.720044E-740.5980344
GGCGATC1252.720044E-740.5980348
CATTAAG19450.038.395232
ATTAAGA22250.034.863483
GTATACG850.002742659334.1159973
AAGATGT23400.034.0795446
Produced by FastQC (version 0.11.5)