FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_H06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_H06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379445
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA60610.43937960556600664No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC46110.3342648673923208No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC41790.3029479247088503No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG39650.2874344392128718No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG36570.2651066189663234No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG34600.2508255131592778No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC33940.24604098024930315No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT26630.19304865362518986No Hit
CATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT26420.19152630224474337No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT23950.17362055029377757No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT23170.16796610230926207No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG23100.1674586518491132No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT23060.16716868015759961No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT22800.1652838641627611No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT21840.15832454356643433No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC21800.15803457187492073No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20430.14810304144057937No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20230.1466531829830113No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA20090.14563828206271362No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT19070.13824400392911643No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA18640.13512680824534504No Hit
CAAGCCAAATTCATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAG18390.13331448517338496No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG18090.13113969748703283No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA17450.12650015042281496No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT16620.12048323782390745No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA16440.11917836521209617No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT16380.11874340767482575No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC16190.11736604214013606No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC15850.11490128276227032No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG15690.11374139599621587No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC15410.11171159415562056No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC15130.10968179231502523No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT15060.10917434185487643No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG14910.10808694801170037No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG14690.10649210370837546No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG14210.1030124434102121No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC13960.10120012033825199No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG13950.10112762741537357No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC13820.10018521941795433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG100.007082215145.06117145
AGCGAAA2350.0111.09861
AGTAGAA7950.0106.7315141
GCAGGTA6850.0100.546112
CAGGTAC6600.098.858743
GGTACTG6800.095.9476555
GCGAAAG2750.094.907832
CGAAAGC3000.086.995693
ATATAAT1100.072.52271
GACAGTA8600.071.650837
AGGTACT9550.071.357734
CTATATT851.6916601E-1068.264084145
GTAGAAA12850.066.0107962
GCCGTCG350.003560834362.16908145
AGCGAGC1251.8189894E-1258.02447145
CCAAATT5100.056.857875
ATATTCA5900.055.2938774
GAGCGAA400.006043881654.392021
TAGATAT1900.053.418413
CATCACC7550.052.831111
Produced by FastQC (version 0.11.5)