FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329175
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3050.071.332331
CTAGACG700.001056811241.4282154
TATACAG8550.034.7657855
TATATAC11950.033.97463
GCGAAAG6500.033.4625132
CGAAAGC6800.033.051193
ATATACT5700.026.7102974
CAGCTCG9450.026.082479
TGTATAT10750.023.6115571
TATATGT7050.022.6239194
ATATACA13300.022.3494324
GACCGTT1952.6168546E-422.3058248
GTATAAA8200.022.1101231
TATATAT6950.021.9071122
GTATATA11450.021.5346811
TATACTT6450.021.3564055
TATACTC2404.364559E-521.1456535
CCTATAC3508.742427E-820.7141083
GTATAGG4952.1827873E-1120.511051
GGTACAA7800.020.4546991
Produced by FastQC (version 0.11.5)