FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_H01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_H01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196022
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3900.033.4684371
GCGAAAG5050.031.586712
CGCGAAG1050.007696578727.628208145
CGAAAGC5500.026.363563
CCTTATA3151.033186E-925.3195062
GTATTAG3801.2732926E-1124.8077471
GTATTAC10250.024.7612021
TATACTC2951.4286343E-824.57625
TACTCCG2202.2293267E-523.0681157
TATATAC15100.023.0462913
TTACGCA1952.6152108E-422.3076294
TATAGAC10750.022.2576122
CAATTCG2003.1044675E-421.7490279
GTATAAA6150.021.2238881
CAGCTCG7900.021.1066519
TAGTACG1750.003659205520.7142264
GTATATA14050.020.6448251
TATACAG9500.020.6052045
ACTCCGT2505.977808E-520.2990938
TTATACT7150.019.2656784
Produced by FastQC (version 0.11.5)