FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_G06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_G06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355252
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC25680.18948505517793No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT13840.10212122911458534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3450.052.5636141
CGAAAGC5100.036.9636733
GCGAAAG5350.035.2363972
TATACAG8050.028.8221265
GGTCGTT2802.6375346E-1028.4833167
GACAGTA4250.027.2952447
TAACGAC6950.026.0811733
GCTATAT3657.2759576E-1225.8353751
TATTACA16100.025.6697062
CCTTATA5700.025.4405822
GTATTAC14350.025.2744881
ATAGACC9250.025.0830423
AGGTCGT3551.546141E-1024.5089576
GCTTATA3001.7067578E-824.1685542
ACGACTG7650.023.6946625
CTTAACG8300.023.5966281
ATTACAT17850.023.5592633
TATATTC7450.023.3575293
CTAGTAC2852.7782153E-722.8965243
TTACATC16500.022.8502714
Produced by FastQC (version 0.11.5)