FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882079
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10260.11631611227565786No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC10150.11506905844034378No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT9830.11144126546488466No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA9460.10724662983701005No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT9340.10588620747121288No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA8990.10191830890430449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2000.094.281741
CGAAAGC2900.064.999773
TAGCGCC400.006050734354.374814
TCAACGT450.00964105648.330428
CCTTATA4150.045.4215242
GCGAAAG4200.044.8807952
CAATTCG1351.0262738E-842.9603779
GTATTAG800.002033122136.2622071
TTATACT5500.035.5907824
GTATTAC5100.035.551181
TAGTACC850.0027426234.1155938
ATATAGT4750.032.0634231
GTCTAAG5750.031.5323521
ATGACGG1453.4819204E-530.010101145
GTAGAAA5400.029.5469841
TACATCA6150.029.4714415
CTAAGGA5500.028.9998973
GTCGGTC1255.155168E-428.9982558
TATTACA6650.027.2555432
TATATAT3750.027.066572
Produced by FastQC (version 0.11.5)