FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890028
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG14440.16224208676580962No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA12540.14089444377030835No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11330.12729936586264703No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10940.12291748124778096No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT9580.1076370631036327No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC9040.1015698382522797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1900.064.900411
CAATTCG4300.050.5779089
CGAAAGC2650.046.5140653
TATATTC7000.042.4685133
CTATACG750.001483801438.6649
GCGAAAG3250.037.9268532
TAGAAAC9550.035.6841772
TTTAAAC7800.033.4648743
GGTCGTT3750.032.8662457
TATATAC6900.032.5757063
AGTAGAA7250.032.015781
GTAGAAA10950.031.7964941
CCTTATA4800.031.7219122
TCGTTTT3900.031.6003849
GTTACGC2301.0022632E-931.524883
AGGTCGT4200.031.0745226
ATATTCA7750.030.874044
ACAATTC7800.030.670968
TACGCAG2651.382432E-1030.0973385
TTACGCA2651.382432E-1030.0973384
Produced by FastQC (version 0.11.5)