FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436040
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT41240.2871786301217236No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA36250.2524302944207682No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC18700.13021921394947214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3450.046.2404371
TATACCG800.00203749936.2478685
CGAAAGC6550.032.0984652
GTATTAC20700.031.1772611
GCGAAAG6700.030.3041131
TTACATC20000.028.9993024
TACATCA20800.028.231515
ATTACAT21700.026.7274683
TATTACA24550.025.9871562
TATAGAC23300.025.825562
TATACAG10300.024.6344725
ACATCAA25250.023.2560586
ATAGACA25750.023.0868243
TAGACAT26900.021.830334
GACATCT27050.021.7085196
TAGGTAC25450.021.085917145
GTACTAT3809.031282E-920.9907251
GTTACGC4251.4715624E-920.4700973
CATCTTA29000.019.9988238
GTATATA16000.019.9411891
Produced by FastQC (version 0.11.5)