FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_F02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_F02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295735
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCATAGGTGACCACTTTGCCCATGGGTTTGAGGAGGTTGTAGCCCTTG30270.23361258281978953No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT22180.1711769767738002No Hit
GTGGAGAACGTGACGGTGTTCGGGACGGCCTCGGCCAGCAAACACGAGGT19380.14956761992228348No Hit
CCTATGGAATGGCCAACCTGCTGACGGGCCCCAAGCGGAACCTGATGGCC18600.1435478705136467No Hit
CTCTAAGACCACTAAAATAACAGAACAGGACTTAAAAAACCCAAGAACCC17970.13868576522205542No Hit
TCCTATGAGAGCACAGAATGGAGAAGCCACGGCTAAGATGCGGTACAAGC15550.12000910680038741No Hit
GACCAGCACAGTGCGGCCCCCGGTGTCTCTCTTCCCGGGGCCCTGAGGGT15260.11777099484076604No Hit
GGGCCACACAGAGTCGATGTGCGGTTTGATGCGGCCCTGGTTGTACAGAG13950.10766090288523503No Hit
GTCCTACCCTAGCTATGATTCAGTTTCATATTTCATTCTGTTTCTCAGAG13020.10048350935955269No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCGT100.00709204144.993596
GGCGTCT1400.067.318458
TCCTATG4850.056.8213461
GTATTAC4450.055.4100531
TATTACA4850.055.3153762
GTATGAC701.6951606E-551.791416145
TATAGAC8650.050.2945562
ATAGACC9650.045.828763
GCGTCTG1751.8189894E-1245.5694169
GCACGGC1053.8261605E-641.4267436
TATACAG700.001056986741.426747
CTAGGGA2150.040.4648863
GATGATA2350.040.1046148
GTAGGAT3700.039.1889723
TTAAGCT4950.036.620197145
CGGTTAG1001.3891964E-436.2609941
ATAGAGT800.00203727936.2483987
GCGAAAG9900.035.887792
CTCTTAA11850.035.4836659
AGACCTC13600.033.5830735