FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_E09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_E09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277654
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAATGACTGAGCCACCCAGGCGCTCCAAAAGAGTTGAAGAACTCTTAC45071.6232433172221543No Hit
TCTTTATCCAAGGACCAGGAACCACTAGGGCCACCTGTTTGTGAGCGAAA31381.1301836098165343No Hit
GCAGGAAACAGCTCCTGTGACTCGAGGAACTTGTGGTCTCCAACAAGAGC27140.9774755631109222No Hit
CCACTAGGGCCACCTGTTTGTGAGCGAAACTACCAGAAATACAATGGCAA26910.9691918718981177No Hit
TCCCAGGACTCTGGGATCATGACCTTAGCCAAAGGCAGACACTTAATGAC20980.7556167028027689No Hit
GAGCAGGGAGCCTGATAACGGGGCTCGATCCCAGGACTCTGGGATCATGA20110.7242827403891174No Hit
CCTGTGACTCGAGGAACTTGTGGTCTCCAACAAGAGCAACTTCTTTATCC16850.6068704214598024No Hit
ATCCCAGGACTCTGGGATCATGACCTTAGCCAAAGGCAGACACTTAATGA16150.581659187333876No Hit
GACCAGGAACCACTAGGGCCACCTGTTTGTGAGCGAAACTACCAGAAATA13980.5035043615435038No Hit
CTCCAAAAGAGTTGAAGAACTCTTACCATATTTCAGGAACTGAAATGGTC13760.4955808308182126No Hit
GGGCAGGGGATGACAAGAGTGCCGCGAAGAGCCAGCAGAGGGAGCCGTCA12250.44119659720371396No Hit
AGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGC12090.4354340294035022No Hit
AAACAGCTCCTGTGACTCGAGGAACTTGTGGTCTCCAACAAGAGCAACTT11990.4318324245283698No Hit
GAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAAC11360.40914231381503596No Hit
GGCCAGAAGCCATCTGGGCAGGGCAGGGGATGACAAGAGTGCCGCGAAGA10760.38753268456424184No Hit
CACTTAATGACTGAGCCACCCAGGCGCTCCAAAAGAGTTGAAGAACTCTT10460.3767278699388448No Hit
ATATAAGCGTGCCCTCTCAGTATTCTGCATAGCAACAAGGCAAGAACCAA10190.36700353677598735No Hit
GGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAACGGGGCTCGAT9720.3500759938628653No Hit
ACCTTAGCCAAAGGCAGACACTTAATGACTGAGCCACCCAGGCGCTCCAA9650.3475548704502726No Hit
GGAAACAGCTCCTGTGACTCGAGGAACTTGTGGTCTCCAACAAGAGCAAC9240.33278829046222996No Hit
GTTTGTGAGCGAAACTACCAGAAATACAATGGCAAGGCCAGAAGCCATCT9160.32990700656212407No Hit
CTCTGGGATCATGACCTTAGCCAAAGGCAGACACTTAATGACTGAGCCAC9060.32630540168699174No Hit
CTCTTACCATATTTCAGGAACTGAAATGGTCACTGGACATATCAGGAAAG8760.3155005870615946No Hit
CACTAGGGCCACCTGTTTGTGAGCGAAACTACCAGAAATACAATGGCAAG8690.312979463649002No Hit
AACTTGTGGTCTCCAACAAGAGCAACTTCTTTATCCAAGGACCAGGAACC8670.31225914267397553No Hit
ACCTGTTTGTGAGCGAAACTACCAGAAATACAATGGCAAGGCCAGAAGCC8670.31225914267397553No Hit
GCTCCTGTGACTCGAGGAACTTGTGGTCTCCAACAAGAGCAACTTCTTTA8380.3018144885360917No Hit
GCCTTGCGCTGAGCAGGGAGCCTGATAACGGGGCTCGATCCCAGGACTCT8260.2974925626859329No Hit
ATCCAAGGACCAGGAACCACTAGGGCCACCTGTTTGTGAGCGAAACTACC8160.2938909578108005No Hit
ATCATGACCTTAGCCAAAGGCAGACACTTAATGACTGAGCCACCCAGGCG7500.2701203656349269No Hit
CTTCAGGAAAGTCCACCCCTGGGCTCTTTGCTTAGGGAAGAGTTAGCCTG7500.2701203656349269No Hit
GCCAAAGGCAGACACTTAATGACTGAGCCACCCAGGCGCTCCAAAAGAGT7420.2672390817348211No Hit
GTCTCCAACAAGAGCAACTTCTTTATCCAAGGACCAGGAACCACTAGGGC7420.2672390817348211No Hit
AAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGA7300.26291715588466225No Hit
TCTTGGAACAGTTTGGCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAG7060.2542733041843446No Hit
ACCCAGGCGCTCCAAAAGAGTTGAAGAACTCTTACCATATTTCAGGAACT6930.2495912178466725No Hit
GCGCTGAGCAGGGAGCCTGATAACGGGGCTCGATCCCAGGACTCTGGGAT6900.24851073638413276No Hit
ATACAATGGCAAGGCCAGAAGCCATCTGGGCAGGGCAGGGGATGACAAGA6890.2481505758966195No Hit
ATCATACACTATGACCTCAAACCAGGTATGTCTAACTTTTAGGCAACTGT6880.2477904154091063No Hit
GATCATGACCTTAGCCAAAGGCAGACACTTAATGACTGAGCCACCCAGGC6840.24634977345905337No Hit
CTCTAATACTGTACAAAACCTAAAGACAAATAAACCGACATTTGACGGTG6750.24310832907143426No Hit
GTATTAGAGTACTGTGAGGGCAATGACCTGGACTTCTACCTGAAACAGCA6680.24058720565884156No Hit
CATCTGGGCAGGGCAGGGGATGACAAGAGTGCCGCGAAGAGCCAGCAGAG6570.23662544029619598No Hit
GCCTGATAACGGGGCTCGATCCCAGGACTCTGGGATCATGACCTTAGCCA6560.23626527980868273No Hit
GGGCAGGGCAGGGGATGACAAGAGTGCCGCGAAGAGCCAGCAGAGGGAGC6430.2315831934710107No Hit
CGCTTACTCTCTGCCCCAGAATCTACAAGTAGTTCGCTCTGAGGCTGGGG6430.2315831934710107No Hit
GTCTAGACTGGGCTAGAGTGTGTCAGTGGTGGGCGGGCCCTCACAGCGCG6420.23122303298349747No Hit
CTCCAACAAGAGCAACTTCTTTATCCAAGGACCAGGAACCACTAGGGCCA6380.2297823910334445No Hit
GGCAAGGCCAGAAGCCATCTGGGCAGGGCAGGGGATGACAAGAGTGCCGC6350.22870190957090478No Hit
CCCTGATACTGTGCTTTTAAAAGTTCTCTTATACCTTGTGTTTAGCAAGG6190.22293934177069302No Hit
GGCCACCTGTTTGTGAGCGAAACTACCAGAAATACAATGGCAAGGCCAGA6140.22113853933312683No Hit
TCTGTAATGTGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAG6030.21717677397048124No Hit
TCATTATTCTGCTCCCAGGCCACCACCAGTGGGTTGCGAGCGCAGGCATA5900.21249468763280915No Hit
GAAATACAATGGCAAGGCCAGAAGCCATCTGGGCAGGGCAGGGGATGACA5800.20889308275767682No Hit
CTTGTGGTCTCCAACAAGAGCAACTTCTTTATCCAAGGACCAGGAACCAC5650.2034906754449783No Hit
GGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAACGGGGC5630.2027703544699518No Hit
GTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAACGG5620.2024101939824386No Hit
TTATAGTACAGTATTGTGCCAGACTGACATACGACATGACATGGAACCAA5560.20024923105735914No Hit
TGATAACGGGGCTCGATCCCAGGACTCTGGGATCATGACCTTAGCCAAAG5550.19988907056984595No Hit
ACCAGAAATACAATGGCAAGGCCAGAAGCCATCTGGGCAGGGCAGGGGAT5410.19484682374466064No Hit
GGGCTAGAGTGTGTCAGTGGTGGGCGGGCCCTCACAGCGCGCTGCCAGCC5380.19376634228212092No Hit
GAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAACGGGGCTCGATCCCA5210.18764361399439589No Hit
GGCAGACACTTAATGACTGAGCCACCCAGGCGCTCCAAAAGAGTTGAAGA5200.18728345350688266No Hit
GATGACAAGAGTGCCGCGAAGAGCCAGCAGAGGGAGCCGTCACGCTTACT5170.18620297204434297No Hit
CCTGTTTGTGAGCGAAACTACCAGAAATACAATGGCAAGGCCAGAAGCCA5110.18404200911926355No Hit
TCCAAGGACCAGGAACCACTAGGGCCACCTGTTTGTGAGCGAAACTACCA5060.18224120668169735No Hit
GTTTGGCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAGTGAGACTCTG4990.1797200832691047No Hit
GTACTGGAGACCCGGCCTGAGTCCTGTGGTAAGCCTGCTTGCTGCTTCCG4990.1797200832691047No Hit
GAACTTGTGGTCTCCAACAAGAGCAACTTCTTTATCCAAGGACCAGGAAC4980.17935992278159146No Hit
AGCGAAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCA4950.17827944131905177No Hit
GGCTTACTGCAGTAATCCTCTCAGCAGACGTCCATATGACGGATGGCAGG4930.1775591203440253No Hit
CAGCTCCTGTGACTCGAGGAACTTGTGGTCTCCAACAAGAGCAACTTCTT4920.17719895985651207No Hit
GTTTTATATGTGTTATATAAGCGTGCCCTCTCAGTATTCTGCATAGCAAC4830.17395751546889293No Hit
GGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAACGGGG4810.1732371944938665No Hit
GTGTTATATAAGCGTGCCCTCTCAGTATTCTGCATAGCAACAAGGCAAGA4810.1732371944938665No Hit
GGACATATCAGGAAAGAAATCTCAGCACACTGTTCTCTTCAGGAAAGTCC4760.1714363920563003No Hit
CCAGAAATACAATGGCAAGGCCAGAAGCCATCTGGGCAGGGCAGGGGATG4730.17035591059376057No Hit
CTCGAGGAACTTGTGGTCTCCAACAAGAGCAACTTCTTTATCCAAGGACC4700.16927542913122087No Hit
CTTCTTTATCCAAGGACCAGGAACCACTAGGGCCACCTGTTTGTGAGCGA4690.16891526864370765No Hit
GATAACGGGGCTCGATCCCAGGACTCTGGGATCATGACCTTAGCCAAAGG4670.16819494766868115No Hit
AAAGCAGGCTTACCACAGGACTCAGGCCGGGTCTCCAGTACCTCACTGCC4530.16315270084349587No Hit
CCACAGGACTCAGGCCGGGTCTCCAGTACCTCACTGCCTGCTTTCCTCTG4520.16279254035598262No Hit
GGGAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATA4510.1624323798684694No Hit
CCTGATAACGGGGCTCGATCCCAGGACTCTGGGATCATGACCTTAGCCAA4460.1606315774309032No Hit
AAGCAGGGGTTCTTGGAACAGTTTGGCGCTGAACAGTTTTAAAACTGAAG4440.15991125645587673No Hit
GCAGTAATCCTCTCAGCAGACGTCCATATGACGGATGGCAGGAGGAGGAG4430.1595510959683635No Hit
AAAGCAGGCAGTGAGGTACTGGAGACCCGGCCTGAGTCCTGTGGTAAGCC4390.15811045401831056No Hit
ACTCTGGGATCATGACCTTAGCCAAAGGCAGACACTTAATGACTGAGCCA4370.1573901330432841No Hit
AGGAACTTGTGGTCTCCAACAAGAGCAACTTCTTTATCCAAGGACCAGGA4370.1573901330432841No Hit
CAGGGGGAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCT4360.15702997255577086No Hit
CCAACAAGAGCAACTTCTTTATCCAAGGACCAGGAACCACTAGGGCCACC4320.1555893306057179No Hit
GTACAGTATTGTGCCAGACTGACATACGACATGACATGGAACCAATTCGT4320.1555893306057179No Hit
AAAGCAGGGAGTGCGGCGTGAGGTAATGCCTTGATCATTATTCTGCTCCC4250.15306820719312525No Hit
GAAATGGTCACTGGACATATCAGGAAAGAAATCTCAGCACACTGTTCTCT4240.15270804670561203No Hit
GCAGGGGGAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCC4210.1516275652430723No Hit
GGCTTACCACAGGACTCAGGCCGGGTCTCCAGTACCTCACTGCCTGCTTT4130.14874628134296644No Hit
ACATTGTACACCCATATCACAATCACACACAAACCCAGGCCCACATGGAA4110.14802596036793994No Hit
TGACAAGAGTGCCGCGAAGAGCCAGCAGAGGGAGCCGTCACGCTTACTCT4100.14766579988042672No Hit
GACTCGAGGAACTTGTGGTCTCCAACAAGAGCAACTTCTTTATCCAAGGA4070.14658531841788702No Hit
GGAACCACTAGGGCCACCTGTTTGTGAGCGAAACTACCAGAAATACAATG4070.14658531841788702No Hit
GAGCAACTTCTTTATCCAAGGACCAGGAACCACTAGGGCCACCTGTTTGT4070.14658531841788702No Hit
GTGGTAAGCCTGCTTGCTGCTTCCGTGTGTGTTTGAGTGAGAGTGCGTGC4030.14514467646783408No Hit
CTCCAGTACCTCACTGCCTGCTTTCCTCTGCAGCTGAACATCTGGCCACA3930.1415430715927017No Hit
GAGTAAGGGTGTGCATCTATTTGTGATAACCTGTTAGTTTTTCTTTCAAT3860.13902194818010907No Hit
GAGCCTGATAACGGGGCTCGATCCCAGGACTCTGGGATCATGACCTTAGC3830.13794146671756935No Hit
ATAATGGATCGCGCCTCTTTCTCCGACATTAATTTGTGCTGTTTCAGGTA3810.13722114574254288No Hit
ATCCATTATTATGCAGATTGTGAATGCTTTAAAGTACTTAAATGAAATAA3790.1365008247675164No Hit
CCAAAAGAGTTGAAGAACTCTTACCATATTTCAGGAACTGAAATGGTCAC3780.13614066428000315No Hit
TATCAGGAAAGAAATCTCAGCACACTGTTCTCTTCAGGAAAGTCCACCCC3740.13470002232995024No Hit
AAAGCAGGCTTACTGCAGTAATCCTCTCAGCAGACGTCCATATGACGGAT3700.1332593803798973No Hit
GGCCTTGCGCTGAGCAGGGAGCCTGATAACGGGGCTCGATCCCAGGACTC3670.13217889891735757No Hit
AGCGAAAGCAGGAAACAGCTCCTGTGACTCGAGGAACTTGTGGTCTCCAA3660.13181873842984432No Hit
GTTAACAACTGTGCCCACCAGGCAGTAAATGGGAACCATCTTCCGAACAC3650.13145857794233112No Hit
CAAGAGTGCCGCGAAGAGCCAGCAGAGGGAGCCGTCACGCTTACTCTCTG3650.13145857794233112No Hit
GTTCTCTTCAGGAAAGTCCACCCCTGGGCTCTTTGCTTAGGGAAGAGTTA3640.13109841745481787No Hit
GCCAGAAGCCATCTGGGCAGGGCAGGGGATGACAAGAGTGCCGCGAAGAG3630.13073825696730462No Hit
ACTCTTACCATATTTCAGGAACTGAAATGGTCACTGGACATATCAGGAAA3600.12965777550476493No Hit
CTTCAGAGTCACCTCGGTTTGGGATTACAAATCTTTGACCAAGGACCACA3600.12965777550476493No Hit
GAAGAACTCTTACCATATTTCAGGAACTGAAATGGTCACTGGACATATCA3580.12893745452973845No Hit
GAGTTGAAGAACTCTTACCATATTTCAGGAACTGAAATGGTCACTGGACA3560.12821713355471198No Hit
GTTTTAAAACTGAAGATCGCTGCAGTGAGACTCTGGCATTCCCTTCCCCC3540.1274968125796855No Hit
GTCCTGTGGTAAGCCTGCTTGCTGCTTCCGTGTGTGTTTGAGTGAGAGTG3480.1253358496546061No Hit
CGTATACATGTTACAGCACCAGTGTGTTAACCAACGGCCATACCCATGCA3380.12173424477947374No Hit
AAACTACCAGAAATACAATGGCAAGGCCAGAAGCCATCTGGGCAGGGCAG3370.1213740842919605No Hit
TCACTGGACATATCAGGAAAGAAATCTCAGCACACTGTTCTCTTCAGGAA3340.12029360282942078No Hit
TTACAGCACCAGTGTGTTAACCAACGGCCATACCCATGCAAGTTTATTGT3310.11921312136688109No Hit
AACAAGAGCAACTTCTTTATCCAAGGACCAGGAACCACTAGGGCCACCTG3290.11849280039185461No Hit
GAGATAGAGAGAGAGAGATGGGAGAAAGAGAAGGAGAGAGAGATGGGAGA3240.11669199795428843No Hit
GTGTATGATGGGAGGTTTTATTTCATTTAAGTACTTTAAAGCATTCACAA3190.11489119551672225No Hit
TACAATGGCAAGGCCAGAAGCCATCTGGGCAGGGCAGGGGATGACAAGAG3130.11273023259164283No Hit
CAACAAGAGCAACTTCTTTATCCAAGGACCAGGAACCACTAGGGCCACCT3100.11164975112910314No Hit
AAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAACGGGGCTCGATCCCAG3100.11164975112910314No Hit
GAGCCACCCAGGCGCTCCAAAAGAGTTGAAGAACTCTTACCATATTTCAG3080.11092943015407666No Hit
GCTACATAGTGTGAGTAATGGGAAAAACAAAGCTGAGCTTGCCAGAGCAG3060.11020910917905018No Hit
GTGTAAGTGTTGCAGCCATGGCAACGGCTGCCGCATTTGGCATGTTGCTG3050.10984894869153695No Hit
GAACCACTAGGGCCACCTGTTTGTGAGCGAAACTACCAGAAATACAATGG3040.10948878820402372No Hit
AGCCAAAGGCAGACACTTAATGACTGAGCCACCCAGGCGCTCCAAAAGAG3000.10804814625397076No Hit
GGGTGGATCTGTCTGATCACAAGTGCACGTATACATGTTACAGCACCAGT2980.1073278252789443No Hit
GATAGAGAGAGAGAGATGGGAGAAAGAGAAGGAGAGAGAGATGGGAGAAA2980.1073278252789443No Hit
GGATATGGGTGCCAGGAGAGGAAGGAAGAGAGAGAATGAGAAGGGGAGGA2910.10480670186635165No Hit
GTCCACCCCTGGGCTCTTTGCTTAGGGAAGAGTTAGCCTGGTATCCCCAG2900.10444654137883841No Hit
CCCATATCACAATCACACACAAACCCAGGCCCACATGGAAACACACACAC2880.10372622040381195No Hit
CCATATTTCAGGAACTGAAATGGTCACTGGACATATCAGGAAAGAAATCT2870.10336605991629871No Hit
TCTCAGCACACTGTTCTCTTCAGGAAAGTCCACCCCTGGGCTCTTTGCTT2870.10336605991629871No Hit
GTGTTAACCAACGGCCATACCCATGCAAGTTTATTGTTTTATATGTGTTA2850.10264573894127223No Hit
TGACTGAGCCACCCAGGCGCTCCAAAAGAGTTGAAGAACTCTTACCATAT2840.10228557845375899No Hit
GTCTATAGAGAACATACGCAGACTCTGAAGAACAGCTCAGGACAACAACA2840.10228557845375899No Hit
GGATGGGGGTCATGGGGTGGTGAATGCATATCTGTAATGTGTACGTGTGT2820.10156525747873253No Hit
GCAGGGAGCCTGATAACGGGGCTCGATCCCAGGACTCTGGGATCATGACC2800.10084493650370605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG100.0070783873145.066671
GGATTAA100.0070783873145.066671
CGTCACC100.0070783873145.06667145
GTTAACA950.099.256141
AACTGTG1000.094.242397
TTAACAA1000.094.242392
GCAGGAA5400.089.995061
AGGGGTT1200.084.57655
AGGAAAC5600.080.2613753
GGGTTCT1300.078.070627
CGAAAGC5500.076.448383
ACAACTG1500.072.494155
GTGGATC702.8048817E-972.494143
GGTGGAT754.840331E-967.661212
GCGAAAG6500.064.687092
AGGGAGT902.8194336E-1064.439246
GGATCTG808.06358E-963.4323775
CGCGGCC350.003557306262.17143145
ACTGTGC3050.061.7982868
AACAACT1550.060.801544
Produced by FastQC (version 0.11.5)