FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_E06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_E06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185584
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG52470.4425667013050109No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT33660.28391071404472396No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA31140.26265536646918314No Hit
GTATGAAAAGATCTCCAGAAGGAGTGCTCGATCTTGCCCACGCTCTTGAT24890.20993873061714735No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA24800.20917961106087804No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT17000.14338924951753734No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG16920.1427144765786313No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT15600.13158072308668134No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC15310.12913467118314687No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA15310.12913467118314687No Hit
CCCCAAGTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGT13720.11572355902238897No Hit
CCCTGTGGGCAGGGGACGAGCCCGCCCGATGGTCGGCAGCAAGGGGTCTC13690.1154705191702992No Hit
GTGCAGATACTGGGTGTTTTTTCCCCTCGGGACCTGTTTCACGATGCATC13680.11538617255293594No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA13560.11437401314457685No Hit
TCCCTGGACAGGCCGGCTGGGACATCACAGCAGGTGCTACAGCCTCCCGA13270.1119279612410424No Hit
GGCCTGTGCTGCTCGACACGCGGAGGAACAGCAGCAACAGGACCCCGACA12740.10745759052078975No Hit
GTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATAGAATGATGGTTA12050.10163767392272499No Hit
GTATAGCCCAGTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGC12030.10146898068799849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTA1600.063.434779
AGCGAAA6400.061.1873051
CTATAGT400.006052192854.3726584
ATAAGAC400.006052192854.3726583
TAGACCT5050.050.245364
TTCAACG2100.048.3312537
TATAGTC604.946505E-448.3312537
CGAAAGC8650.046.0962833
GTACACC4250.046.0568433
GGTCGCA951.9212002E-645.7894332
GCGAAAG8650.045.2600752
GTCGCAT1351.0239091E-842.97381
GTTATAT2200.042.8517151
ACCTTAC1601.0077201E-940.791541
ATAGACC6250.040.598253
TTACATC5100.039.802214
ATCTATC750.001483811238.6650058
TTGCGCA750.001483811238.6650057
ATTGCGC750.001483811238.6650056
TATATGT11300.037.8523524
Produced by FastQC (version 0.11.5)