FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATGGCTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA22.4096385542168677No Hit
CATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGT11.2048192771084338No Hit
CCACTATATGATGCAATCAAATGCATGTAGACATTCTTTGGCTGGAAAGA11.2048192771084338No Hit
CACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCTC11.2048192771084338No Hit
GACCAGATGGCTCTTCAATTGTTCATAAAAGAATAAAGATAAACATATAT11.2048192771084338No Hit
ATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGTGGAGA11.2048192771084338No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT11.2048192771084338No Hit
GACCATGGGCTGGCATTACCACAGCATTGTTACAGAATCAATCTCTTTAT11.2048192771084338No Hit
ATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCA11.2048192771084338No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA11.2048192771084338No Hit
GGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATC11.2048192771084338No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG11.2048192771084338No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG11.2048192771084338No Hit
TAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGA11.2048192771084338No Hit
GAAGCACCCCGCACTCAGAATGAAGTCGATGATGGAAATGAGATACAAAA11.2048192771084338No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT11.2048192771084338No Hit
GAAGATGGTCACACAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATA11.2048192771084338No Hit
GTATGGATAGCAAATAGTAGCATGGCCACAACTACTTCATTGCATGTGGG11.2048192771084338No Hit
CCTCATGCTTGCGATATGTTCAATCGGGGCAACCTCTTCTCCTATTTCAT11.2048192771084338No Hit
ACAAACTAGTTTTCATCCACATGGACTGCTCAGAATATCTATAGATCATA11.2048192771084338No Hit
GTACAGTCTATTTAGATAAGTTCCATAAACTGATTTACGATATAGAGAAT11.2048192771084338No Hit
ATTCCGGTGCGAACAAGCGCTCTTGTTCTCTGATATGTGGCATCATTCAG11.2048192771084338No Hit
ATTGAGAAAATAAGGCATCTTCTAATAGATGGAACAGCATAACTGACTCC11.2048192771084338No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA11.2048192771084338No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA11.2048192771084338No Hit
AAGCAATATTCCAACCACAGGACTCCACTTCTGACTCTAAAAATAAAGCC11.2048192771084338No Hit
AAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTG11.2048192771084338No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG11.2048192771084338No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT11.2048192771084338No Hit
GATAATCATCATCCATTAGCTCCCATTTTAAGCAGACTTCAGGAATGTGA11.2048192771084338No Hit
AACTAACTCAAGGTCGCCAGACTTATGATTGGACATTAAACAGAAATCAA11.2048192771084338No Hit
AATCTGGACCCCTCTTGTGGAAAGCTTTCCTCTTGGAATCACTTTCTTTC11.2048192771084338No Hit
GAACTCACCCCCGCGGCGATCGACGAGCAGGCCCTGGACGACGAGATCGG11.2048192771084338No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT11.2048192771084338No Hit
ACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTAT11.2048192771084338No Hit
AAATTGAAGATCCATCTGCGACTAAGCTGGTAACATTTCATTGTTTATCA11.2048192771084338No Hit
CTTAAAGGATGTAATGGAATCTATGAACACAGAGGAAATAGAGATAACAA11.2048192771084338No Hit
ATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGGCT11.2048192771084338No Hit
GGATTCAGGGGATTACAAAGTCTTCCCCGATAATCATCATCCATTAGCCC11.2048192771084338No Hit
GATTAGGAGAAACAAGGCATATGTTCAGTAGGGACAAGATAAAGACATTA11.2048192771084338No Hit
ATGTTCGAGAGTAAAAGAATGAAGATTCGAACACAAATACCAGCAGAAAT11.2048192771084338No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA11.2048192771084338No Hit
AGATTGCAGCACTTCTGGTTCATCTGTTCATCCTCAAGAATTCCCCTTTG11.2048192771084338No Hit
GTCCCCGCCTGCGTGTAGTCCCTCATTGTGTGTTGAAGTCCCTAGGAGCG11.2048192771084338No Hit
GGGCTTGGGTGACGGTCGGGCAGGATTCCTAGAAGTCACAGAAACGGTGA11.2048192771084338No Hit
TCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGAGTTGGAACAA11.2048192771084338No Hit
CTCCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAG11.2048192771084338No Hit
CATGGTACCTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGAG11.2048192771084338No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA11.2048192771084338No Hit
GTAATTGGGATTTATGCCTTTCTCATGTGGTTAGACTATGTTAGTCTCTT11.2048192771084338No Hit
AAGAAGAAGTGCTAACGGGCAACCTCCAAACACTGAAAATAAGAGTACAT11.2048192771084338No Hit
ACTAGAAAAGGTGGACAACATGAATAAACAAATAGGGAATTTCAGCAGAG11.2048192771084338No Hit
CTATTGAAATGATAATAAATTTACTGTATTTGCAAGAAGTCAGATCGACA11.2048192771084338No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG11.2048192771084338No Hit
CAACATGAGCAAAAAGAAGTACTATATAAATAAGACAGGAACATTTGAAT11.2048192771084338No Hit
AGTAAAGACGACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCAT11.2048192771084338No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACATTGGA11.2048192771084338No Hit
AAGTTTGATTATCGCTGCTAGAAACATAGTAAGAAGAGCAGCAGTGTCAG11.2048192771084338No Hit
TGCCGCCCCTGAGTTACACCGTTCCCACCCCACTGCAGTATTTCGCTGCG11.2048192771084338No Hit
GCAAATACATAACACAAATAAGAAAAAAGTTATTTTTGAGACCTACTTTG11.2048192771084338No Hit
GGACAGCGCATCCACCCGCACTACTAATCACGAGTCAGAGGGCACTGCCG11.2048192771084338No Hit
GGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGCGATAATC11.2048192771084338No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA11.2048192771084338No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT11.2048192771084338No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA11.2048192771084338No Hit
CGCGTCGTTAGTCGCGTTGGTTAGGGACATCTGTGAAGCTGAGGTCCGGA11.2048192771084338No Hit
TTCGAGCCTAAATCATGGCCATCAGAAGTACTTTCGTCTATGTCTGGATG11.2048192771084338No Hit
GCAACAGCTATTCTCAGAAAGGCAAACAGGAGATTGATACAGTTGATAGT11.2048192771084338No Hit
AGCTAATCTTTCAGTTTTGGTGGGTTCACTTTCAAAAGGACAAGCGGATC11.2048192771084338No Hit
CATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCA11.2048192771084338No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG11.2048192771084338No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT11.2048192771084338No Hit
GTGATAAAGAACAACATGATAAACAATGACCCTGGACCTGCAACGGCCCA11.2048192771084338No Hit
GCAACAAGAAGTCCGATATAAATAAGACAGGTACATGTGAATTAACAATA11.2048192771084338No Hit
CAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCGATGAGGACC11.2048192771084338No Hit
AAGAAGTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTT11.2048192771084338No Hit
TGGGATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATT11.2048192771084338No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG11.2048192771084338No Hit
TCTTCTTCTTTCTTGACTGATGATCCGCTTGTCCTTTTGAAAGTGAACCC11.2048192771084338No Hit
GCCATCAGCGGTCAAAGTTCCTGCTGATGGATGCTCTGAAATTAAGTATT11.2048192771084338No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG11.2048192771084338No Hit
GTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGA11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGC50.0145.01
TGACACT50.0145.08
TGCTGAC50.0145.05
GTCTGCT50.0145.02
CTGACAC50.0145.07
GACACTG50.0145.09
TCTGCTG50.0145.03
GCTGACA50.0145.06
CTGCTGA50.0145.04
AAAAAAT50.0145.0145
Produced by FastQC (version 0.11.5)