FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533765
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG24580.16025923136856038No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA18360.11970543075373347No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC17470.11390271651784986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3750.034.8069651
CGCCAAT3450.033.6222424
TATAGCG1153.163403E-431.5208517
TATACAG16400.029.1760085
TATAGAC11650.028.0035062
CGAAAGC6150.027.113062
CAGCTCG14650.026.7219289
ATAGACC7150.026.3628943
GTACGAT1100.00967602626.3620369
GTATTAC12400.025.146161
GTATATA26750.024.668491
GTCTAGA5650.024.385411
CGGTTCG1550.001806822623.3917771
GTATTAG5050.022.9748951
GCGAAAG7500.022.2377831
ATACAGC19150.021.9576076
GTATAAG6750.021.485781
TATATAT17950.021.4060842
GTATAGG5800.021.2542531
TATATAC27250.021.0177563
Produced by FastQC (version 0.11.5)