FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_C09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_C09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613630
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA47260.2928800282592664No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT35250.21845156572448454No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC34390.21312196724156096No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC27390.16974151447357821No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG25840.16013584278923917No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT22890.14185408055130358No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC22390.13875547678216194No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG20940.12976952585165125No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20650.1279723356655491No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA18170.11260326097060663No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG17890.11086804285988733No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT17630.1092567688999337No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA17450.1081412715430427No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC16540.10250181268320496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3200.088.3780751
GCGAAAG4550.062.14062
CGAAAGC5000.057.9978943
TAGGACG858.930492E-751.1746144
AGCTATC7200.044.3039479
AGTAGAA14450.042.6560861
CCCTTAT21100.039.8662571
TTATACT23150.039.458614
GTAGAAA16000.039.4204442
GGTCGTT3900.039.0370457
CCTTATA22450.038.7513772
CTCGTAC750.001483921238.665263
CGATGTC750.001483921238.665265
GAGCTAT8550.037.3085868
ACTCGTA800.00203734836.2486842
AGGTCGT4250.035.8222276
ACTGATC7700.035.777928
GGTACTG7800.035.319235
CAATTCG5600.032.3648959
TAAGATG15750.032.221055
Produced by FastQC (version 0.11.5)