FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_C04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_C04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131138
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG4420.33704952035260566No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA3330.25393097347832055No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC3000.22876664277326175No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT2900.22114108801415305No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC2800.2135155332550443No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT2640.20131464564047036No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG2610.19902697921273774No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG2540.1936890908813616No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA2490.18987631350180725No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC2450.18682609159816377No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC2400.18301331421860942No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG2390.18225075874269853No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG2320.17691287041132242No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA2200.16776220470039196No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC2070.15784898351355062No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2030.15479876160990713No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG1950.14869831780262013No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG1820.1387850966157788No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT1710.1303969863807592No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA1700.12963443090484833No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG1670.1273467644771157No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT1630.12429654257347222No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA1630.12429654257347222No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT1590.12124632066982874No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG1570.11972120971800698No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT1570.11972120971800698No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA1530.1166709878143635No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT1470.11209565495889826No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC1460.11133309948298739No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG1370.10447010019978954No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC1360.10370754472387865No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC1350.10294498924796779No Hit
TTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG1330.10141987829614604No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT1330.10141987829614604No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT1330.10141987829614604No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG1320.10065732282023518No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA1320.10065732282023518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG151.2283212E-4144.978261
CGAAAGC203.860788E-4108.7336962
TCTACAC301.5954425E-596.652173
TACTCTA301.5954425E-596.652177
GGTACTG406.0931234E-790.611415
GTGTTTT259.3740143E-486.986959
TAGATAT259.3740143E-486.986953
TTTAAAC608.221832E-1084.570653
CAGGTAC353.4286742E-582.844723
ACTCTAG353.4286742E-582.844728
CTACACT353.4286742E-582.844724
AGTAGAA353.4286742E-582.844721
GCACTAG451.2279925E-680.543471
CAATTCG651.551598E-978.0652169
ATATTTA300.001933129272.489136
TTTTAAA702.7903297E-972.489132
GCAGGTA451.1908906E-464.4347842
GACACAT350.003561712362.1335371
GAAAGCA350.003561712362.1335373
TTATAGG1300.061.3369561
Produced by FastQC (version 0.11.5)