FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534662
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT48750.911791000669582No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC34250.6405916261114498No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG28040.5244434801800015No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC25600.47880717163366765No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG20060.3751903071473192No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG18610.348070369691506No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC18530.3465740972801508No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA16450.30767101458491536No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC16060.3003766865795587No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT15960.2985063460653647No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA15130.28298251979755434No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT13670.25567554829032174No Hit
TGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGA12570.23510180263418756No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC12430.23248332591431597No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT11950.2235056914461847No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT11270.2107873759496654No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG10680.1997523669159207No Hit
TTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAG10190.19058769839637005No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC9860.1844155746995298No Hit
GTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAG9730.18198413203107758No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA9480.17730828074559254No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA9320.1743157359228821No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG8910.16664733981468666No Hit
GATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAA8690.16253259068345982No Hit
CTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTA7920.14813096872416592No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA7910.14794393467274652No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG7800.1458865601071331No Hit
GAATGGAACATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATC7730.14457732174719728No Hit
GTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTAT7690.1438291855415197No Hit
GGGAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTA7530.14083664071880925No Hit
ATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACTCG7440.13915333425603466No Hit
CTTTTGGGCAGCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGG7350.13747002779326006No Hit
GAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAA7150.13372934676487203No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT7090.1326071424563556No Hit
GTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCACCCGCATAAT6320.11820552049706169No Hit
ATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTT6310.1180184864456423No Hit
GTCGAAGAGCAATACTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTA6150.11502594162293187No Hit
GTTCCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAA6120.11446483946867367No Hit
ATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAG6010.11240746490306025No Hit
TGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTGG5830.10904085197751102No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA5520.10324279638350958No Hit
CATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTA5520.10324279638350958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAA10100.066.777971
AGCGAAA1200.066.478781
TGTACCG350.003565213462.144545
GACTACG350.003566534762.138737
AGATGTA11850.059.342057
GGTCGGT753.7595964E-757.996157
TAAGATG12800.056.642165
GATTAGC5450.054.5580671
TTAAGAT13300.054.5127564
CATTAAG12850.053.0364572
GTCGGTC858.921015E-751.173078
ATTAAGA13350.051.0500763
AGGTCGG901.3221616E-648.334646
GCGAAAG1950.044.6291161
CGAAAGC2000.043.501182
AAGATGT18550.039.475466
GTAGAAA5550.039.2012521
ATTAGCA7700.038.6049422
TAGCACA8500.036.677464
GTCTAGT10950.036.426731
Produced by FastQC (version 0.11.5)