FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_B01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_B01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461846
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA10420.22561633098478717No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT10140.21955370404853564No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAAGATCAAAT7940.17191877812084547No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG5910.12796473283302226No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA5490.11887079242864505No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT5050.109343807243107No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA5030.10891076246194618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2100.069.072271
GCGAAAG2400.060.4120672
CGAAAGC2700.053.6996153
CAGCTCG4350.049.996199
TATACAG4850.046.3366785
GAGCCGT657.335219E-444.6119846
TATACTC657.335219E-444.6119845
CCATCGC700.001056445641.4254159
TTACATC2500.040.596914
TACATAC4150.040.1776586
CTCCGTA1157.1380964E-637.8232087
CAATAGA1604.5834895E-836.247242
ATATATA5650.034.643382
TACAGCT6550.034.3103647
ACAGCTC7100.033.694628
TATATAC13000.033.458993
AATAGAC1751.00786565E-733.1403353
GTATTAC3100.032.7536281
CAATAGG900.003619889332.2337271
GTATCTA900.003627577832.219776
Produced by FastQC (version 0.11.5)