FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_A12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_A12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486327
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC39220.8064532711529485No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT36300.7464113651925557No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC25920.5329747268812959No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG24410.5019256590730106No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG21440.4408556382845287No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG19270.39623545474546956No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC18880.3882161590863761No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG18200.37423379742436674No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC15650.32179994119183186No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA14910.3065838417361158No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA14810.304527612079938No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC14800.3043219891143202No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT13910.2860215451743374No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT13870.2851990533118663No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT13270.2728616753747993No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA12940.2660761175094124No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA12850.2642255108188523No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA12140.24962628025998967No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC11910.24489695205078066No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG11800.24263509942898503No Hit
TGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGA11440.2352326726667448No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA11410.23461580376989144No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG10720.22042781914226434No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT10620.21837158948608654No Hit
TTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAG10100.20767919527396173No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT9240.18999562023083236No Hit
GTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTAT9180.18876188243712563No Hit
ATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACTCG9030.18567753795285888No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG8730.17950884898432537No Hit
GTTCCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAA8700.178891980087472No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC8610.17704137339691195No Hit
GTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAG8590.17663012746567638No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA8350.17169517629084957No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG8340.17148955332523177No Hit
GAATGGAACATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATC8170.16799396290972946No Hit
GTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCACCCGCATAAT8150.1675827169784939No Hit
CTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTA8140.1673770940128761No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT8050.16552648732231606No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA7930.16305901173490264No Hit
GATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAA7930.16305901173490264No Hit
CCTAAATTCCGTATGGGAGCTTCCTGGTAACTCGTTCTGTTCCCTAATAC7710.1585353064913114No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG7430.15277786345401345No Hit
GGGAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTA7390.15195537159154232No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC7390.15195537159154232No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT7370.15154412566030676No Hit
ATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTT7030.1445529448293021No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC6940.14270233813874206No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT6750.13879550179200414No Hit
GAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAA6540.1344774195140307No Hit
CTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGT6370.13098182909852835No Hit
CTTTTGGGCAGCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGG6330.13015933723605722No Hit
TTATCAGCCATTAGCTGGTTTCCTGTGTCCCAGCAAATGCTGAGGTAAGT6290.12933684537358608No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT6270.12892559944235052No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG6180.12707499275179046No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC6010.12357940233628814No Hit
GTCGAAGAGCAATACTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTA5740.11802758226460797No Hit
TGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTGG5720.1176163363333724No Hit
ATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAG5710.11741071336775462No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA5580.11473761481472344No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA5560.11432636888348785No Hit
ACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGAGTTAC5470.11247576219292782No Hit
GTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACT5370.11041953253674998No Hit
TCATTTGGCTGGGAATGGAACATTTACCATATGTATGAGTTACTAATTTC5360.11021390957113217No Hit
CATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTA5240.10774643398371876No Hit
GAACATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCA5080.10445646653383424No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA4980.1024002368776564No Hit
CCATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGT4910.1009608761183319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1400.088.049961
CTACGGT451.1922892E-464.454865145
TAGGACG350.003566022562.140134
TAAGATG9150.061.0082475
AGTAGAA8000.059.8221741
CAGGTAC1950.059.4845663
CGAAAGC2100.058.6878973
TTAAGAT9200.058.3126564
GCGAAAG2200.056.0202642
GTAGAAA8650.055.3154872
AGATGTA10650.052.415547
GAGCGAA701.6930642E-551.794091
AAGATGT11700.050.19016
CGGATAT450.00963785548.331219
GCAGGTA2400.048.3312072
GATTAGC7000.045.57881
ATTAAGA12050.044.5208633
GGTCGTT4300.043.835287
AGGTCGT4300.043.835286
TAGAAAC11200.042.721343
Produced by FastQC (version 0.11.5)