FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_A07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_A07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493889
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC24390.16326514218927912No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT22160.1483376609641011No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA17620.11794718349221395No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC17360.11620675967223804No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA16930.11332836643150863No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG16770.11225733638844652No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC16050.10743770119466707No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG15110.10114539969167723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3050.064.197331
ACCGGTA400.00605314454.3711979
CAATTCG5700.047.0581139
TATACGT2700.045.644964
CGAAAGC4550.044.6122633
GCGAAAG5050.038.7609632
ACGGGCG951.0273285E-438.16928145
GTATTAC13250.037.2174031
TAACGAC5300.035.563553
GGCGGTT8300.034.9373137
CCTTATA9550.034.9201932
TACGTGC3650.033.764766
AGTAGAA11700.032.850581
TATTACA15450.032.84672
CGTGCTA3550.032.673778
ACTGATC9000.032.2199678
GGATAGC8350.032.12355
ATACGTG3850.032.010755
CTTAACG5900.031.9576151
GTAGAAA13400.031.3889061
Produced by FastQC (version 0.11.5)