FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R1_A01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R1_A01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7548
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT1171.550079491255962No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT1071.4175940646528882No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA821.086380498145204No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT550.7286698463169051No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG530.7021727609962904No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG480.6359300476947536No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG420.5564387917329093No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG420.5564387917329093No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG380.50344462109168No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG360.47694753577106513No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC360.47694753577106513No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT310.41070482246952833No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA310.41070482246952833No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC310.41070482246952833No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG300.39745627980922094No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG290.3842077371489136No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG290.3842077371489136No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG280.37095919448860626No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG280.37095919448860626No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA270.3577106518282989No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA270.3577106518282989No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA270.3577106518282989No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC240.3179650238473768No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG240.3179650238473768No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC240.3179650238473768No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT240.3179650238473768No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT240.3179650238473768No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC230.3047164811870694No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG230.3047164811870694No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT220.29146793852676206No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT220.29146793852676206No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA220.29146793852676206No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC220.29146793852676206No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT220.29146793852676206No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA220.29146793852676206No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG210.27821939586645467No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC210.27821939586645467No Hit
GTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTC210.27821939586645467No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT210.27821939586645467No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT210.27821939586645467No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC200.2649708532061473No Hit
GCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAACCAAGTGGGT200.2649708532061473No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG200.2649708532061473No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA190.25172231054584No Hit
CTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATA190.25172231054584No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC190.25172231054584No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG190.25172231054584No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA180.23847376788553257No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACTGGATTCCCAAG180.23847376788553257No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC170.22522522522522523No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA170.22522522522522523No Hit
ATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGT170.22522522522522523No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG160.21197668256491786No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT160.21197668256491786No Hit
CATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAAC160.21197668256491786No Hit
GTTCTATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATG160.21197668256491786No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA160.21197668256491786No Hit
GTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCAC160.21197668256491786No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA160.21197668256491786No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG150.19872813990461047No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG150.19872813990461047No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA150.19872813990461047No Hit
GGTATGCACAAACTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAA150.19872813990461047No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC150.19872813990461047No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT150.19872813990461047No Hit
TGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAA150.19872813990461047No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA150.19872813990461047No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT150.19872813990461047No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG150.19872813990461047No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG140.18547959724430313No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA140.18547959724430313No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA140.18547959724430313No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA140.18547959724430313No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT130.17223105458399576No Hit
CCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAACCAAG130.17223105458399576No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG130.17223105458399576No Hit
GTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGA130.17223105458399576No Hit
ACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCT130.17223105458399576No Hit
CAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAA130.17223105458399576No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT130.17223105458399576No Hit
GGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGA130.17223105458399576No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG120.1589825119236884No Hit
CACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTG120.1589825119236884No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG120.1589825119236884No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT120.1589825119236884No Hit
GATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCAT120.1589825119236884No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC120.1589825119236884No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT120.1589825119236884No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC120.1589825119236884No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTT120.1589825119236884No Hit
TACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA120.1589825119236884No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA120.1589825119236884No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT120.1589825119236884No Hit
CTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTG120.1589825119236884No Hit
GACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTC110.14573396926338103No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA110.14573396926338103No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG110.14573396926338103No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT110.14573396926338103No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT110.14573396926338103No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC110.14573396926338103No Hit
TTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAG110.14573396926338103No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT110.14573396926338103No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA110.14573396926338103No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC100.13248542660307366No Hit
TTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAA100.13248542660307366No Hit
GATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGG100.13248542660307366No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC100.13248542660307366No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA100.13248542660307366No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGTTTGTGCATACCCACTTGGTT100.13248542660307366No Hit
GAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAA100.13248542660307366No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT100.13248542660307366No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA100.13248542660307366No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG90.11923688394276628No Hit
GAACAGATGTACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAG90.11923688394276628No Hit
TCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTG90.11923688394276628No Hit
CACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGT90.11923688394276628No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA90.11923688394276628No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG90.11923688394276628No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG90.11923688394276628No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC90.11923688394276628No Hit
ATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCA90.11923688394276628No Hit
CCCAAGAGGAATCGTTCTATTCTCAACACAAGCCAAAGGGGAATTCTTGA90.11923688394276628No Hit
CTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGT90.11923688394276628No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG90.11923688394276628No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG90.11923688394276628No Hit
CCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTA80.10598834128245893No Hit
GTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACAC80.10598834128245893No Hit
TCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCA80.10598834128245893No Hit
CTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGAC80.10598834128245893No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC80.10598834128245893No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC80.10598834128245893No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG80.10598834128245893No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA80.10598834128245893No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT80.10598834128245893No Hit
ACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACT80.10598834128245893No Hit
AGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATG80.10598834128245893No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA80.10598834128245893No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA80.10598834128245893No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG80.10598834128245893No Hit
AACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAG80.10598834128245893No Hit
ACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAG80.10598834128245893No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT80.10598834128245893No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA80.10598834128245893No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG80.10598834128245893No Hit
GAATCGTTCTATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAAC80.10598834128245893No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCAAA100.0069521423145.01
TCAAATA100.0069521423145.03
CAAATAT100.0069521423145.04
TAGAAAC309.9777026E-8120.833323
GTAGAAA309.9777026E-8120.833322
AGTAGAA309.9777026E-8120.833321
ACAAGGC256.0525963E-6116.08
AGAAACA352.5017835E-7103.571424
CAAGGCA301.4977417E-596.666669
AAACAAG405.5429155E-790.6256
GAAACAA451.1173543E-680.555565
CCCTTAT300.001861434972.499991
CCTTATA350.003430090862.1428532
AACAAGG659.9207955E-655.769237
TATACTG400.005820287454.3755
TTATACT400.005820287454.3754
TTTTTCA200.006001198729.015-19
GGACAAG200.006001198729.025-29
GACAAGT200.006001198729.025-29
AAGGCAT200.006001198729.010-14
Produced by FastQC (version 0.11.5)