FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755152
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT22940.3037799012649109No Hit
TATGTATAGTGTCCAGGAGGCACCCAGGTGTGAGAGTGAAACTATGGGGT21280.2817975718795686No Hit
GATATGTATAGTGTCCAGGAGGCACCCAGGTGTGAGAGTGAAACTATGGG19240.2547831429963769No Hit
CTACAAGAAGGGGGGCGACTGAGGCTGAGATTCGGGGGGATAATGTCCTA17360.22988749284912177No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA12430.1646026230480751No Hit
TCTCTAGTCCCTATCCCTTTGCTCTGACATTGTTGATTTCCTCCAGCCAT10730.14209059897874865No Hit
GTGTAGGGTGACCTGACTCCATCCCCACTCCAGAACTTGGCCTCAGCACC10120.13401275504799035No Hit
CTCTTGCCCCATGCTGTGGTAGTCTTTGAAACCTGGTATGCATTTTAACA9890.13096701061508148No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC9770.12937792656312902No Hit
CTCTAGTGATCACTTTAGCTATTGTTGTCTTGCCTGCACCATCATCTCTG9540.12633218213022013No Hit
CTCCCAGGGGAGCACAGTTCTCCCTGAATGTGGGGGTGTGATGGTGAGAA9510.12593491111723204No Hit
GTCCAGGAGGCACCCAGGTGTGAGAGTGAAACTATGGGGTCAGCCAGGCT9480.12553764010424392No Hit
GTCCTGGGGCAGCGTGGGCCGTGCTTTCAAGAACTGTTTGGTCACTGTGT9080.12024069326440238No Hit
GTCCAGCACGGTAGCCCCAGGTGGCGACTTAACCAGACTTCGTTAAATTT8900.11785706718647371No Hit
CCCTTGAATCCCTCTCTGGGAGAGAAGAGGTTTCTGTTGTTCGAAGCCAC7890.10448227641587388No Hit
GTCCAGGGGTGGGACCACAGAGGTCTTTGTGGCGCTGGAGTGTGGCATTT7860.10408500540288579No Hit
GTATAGTGTCCAGGAGGCACCCAGGTGTGAGAGTGAAACTATGGGGTCAG7620.10090683729898087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3650.089.3924261
TATGCCG350.003566636762.1407855
CTATACC902.0490916E-856.3870124
GCGAAAG6200.052.6192132
CTATAGG657.331856E-444.619811
TAAGGCC855.3412983E-542.6456345
CTCTATA2050.042.437612
TGTAGGG5150.042.23162
CCTTCGT1053.8237504E-641.427199
TTGCGCA1252.711422E-740.61209145
TATACTC1807.2759576E-1140.2764364
ACCTCTT10000.037.6987427
TAGACCT10400.036.2487954
GACGTAT1051.8500471E-434.534092145
TCTATAG2103.765308E-1034.527231
GTATACT2103.783498E-1034.522663
GATAGCT2950.034.4056367
TAGGGTG5800.033.7488754
TAGTGCA1956.9903763E-933.4604232
CTCTTAA11650.032.981739
Produced by FastQC (version 0.11.5)