FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_H08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_H08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177143
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG17790.1511286224358468No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA17180.14594658422978346No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA17130.14552182699977828No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC16830.14297328361974712No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT16340.13881066276569626No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT16080.1366019251696693No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA13490.1145995006554004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3650.035.7612721
CGAAAGC3900.035.320763
GTATTAC15250.032.334961
CAATTCG3050.030.9005439
TATTACA17500.030.2430692
TACATCA16200.029.0884765
TATACAG12950.027.9912855
CAGCTCG11250.027.7101279
ATTACAT16750.027.7016853
TTACATC17350.027.5794454
GCGAAAG4750.027.4738772
TAACGAC5150.026.747764
ACGACTG5500.026.3627036
ACGCCGA1100.00967393926.3627018
GTATATA23200.025.3180541
GGTCGTT2401.5989772E-624.165817
TAGACAG5100.024.165815
GTCCATA3952.3646862E-1123.8659971
TATAAGG3052.0352672E-823.7706582
TATATAC26100.023.6112773
Produced by FastQC (version 0.11.5)