FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815937
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG8630.1057679698310041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1400.077.6995241
CAATTCG3900.042.752229
CGAAAGC3200.033.9831243
GCGAAAG3200.033.9831242
CGTTACG900.003629585332.2190639
TATAGTC1703.0116807E-629.8518452
TCGGACC1050.007709015627.6180323
GTCCTAG3503.6379788E-1226.9358351
ATAGTCC2456.4375854E-826.6316743
ACTGTCG1958.758569E-626.023098
AGTAGAA7150.024.3422281
ACAATTC6700.023.8036378
CTAATAC3052.0343577E-823.7696193
CCTTATA4600.023.6404342
CTATAGT2151.861302E-523.611021
GGACCGA1851.8307203E-423.512656
GGTCGTT1901.7284037E-822.892497
TAGAAAC8450.022.3068733
TACACCG1952.6148214E-422.3068715
TACTGAT5200.022.3055067
Produced by FastQC (version 0.11.5)