FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_G07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_G07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212898
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG16870.13908836522114804No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA15050.12408298142135613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3700.058.8202441
CGAAAGC5950.036.5531653
CAGCTCG9950.031.3304129
TATACAG12300.030.0597785
GCGCTAG1306.460287E-427.9019091
GCGAAAG8200.026.5233362
ATAGTAC6300.026.46723
TACACCG3751.0913936E-1125.1323325
GTATATA21100.025.0984951
GTTACGC2601.1455995E-725.0951543
GTATTAC8150.024.9234261
TATATGT10350.024.5159324
TATATAC25200.023.590333
TACAGCT13100.023.243357
ATACAGC13800.022.5896826
GACCGTT2252.6529602E-522.5546578
TATAGTA6000.021.7491322
TTACGCA3004.558333E-721.7491324
GTATTAA8100.021.4948041
GTCTTAG4751.0913936E-1121.3816741
Produced by FastQC (version 0.11.5)