FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_G03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_G03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243667
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3900.052.0833171
GCGAAAG6100.032.0957072
GTATATA16750.031.6164591
CGAAAGC6650.031.6143783
TCGACAC1204.0602376E-430.2062643
TCGGTAG1050.007711739727.6171553
TACATAC10150.023.5698136
TATATAT12100.023.3718052
TATACAT13050.023.3317364
GTATATG6200.022.2314151
GTTATAC2353.7120757E-521.5942653
GTCTATT4751.0913936E-1121.381571
TATATAC17250.020.5927933
TATACAG12150.020.2866765
ATATATG10150.019.9986293
GTACTAT5453.6379788E-1219.9664081
GTATAGA9900.019.7848951
CATACAT12100.019.7713728
CTATAGG4803.110472E-1019.6475011
TATAAGG5657.2759576E-1219.2511122
Produced by FastQC (version 0.11.5)