FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136938
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT11880.10449118597496082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4500.062.8553541
GCGAAAG5350.052.852712
CGAAAGC5950.047.523023
GTATTAC8500.033.2763631
GTTACGC1050.00771059927.617798
TATAGAC9600.027.1886562
GTATTAG5100.027.019271
GTATATG3005.838956E-1026.592651
GTATAGG3306.184564E-1126.3728751
GTCTAAT3801.2732926E-1124.8113231
TTACATC9650.024.79384
TATTACA12950.024.634252
GTATATA7100.024.515631
ATTACAT12250.023.6744773
TAGACCT6400.022.6572114
CTAATAC4058.058123E-1021.4823933
TACATCA11550.021.3419595
ATATACG1700.003093868321.322569
ATAGACC6650.020.7151663
ACCTTAT3208.4764906E-720.3896988
Produced by FastQC (version 0.11.5)