FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_F09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_F09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064947
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC72080.6768411949139252No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG57130.5364586218844694No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA53330.5007760949605943No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG50320.4725117775814195No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG44950.42208673295478555No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT43070.40443327226613157No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA40380.3791737992594937No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG32010.30057833864032674No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30900.29015528472308955No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG30380.28527241261771713No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC26010.24423750665526078No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT23710.22264018772765215No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC23380.21954144196847356No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC22960.21559758372951895No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT22480.21109031717071366No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT22410.21043300746422122No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT20710.1944697717351192No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG20230.18996250517631394No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG19710.18507963307094155No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC18430.17306025558079416No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG17810.167238369609004No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT17520.16451522939639251No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG17260.1620737933437063No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC17230.16179208918378096No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT16990.15953845590437832No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT16680.15662751291848329No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC16330.1533409643860211No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG15670.14714347286766383No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG15470.1452654451348283No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG14860.13953746054967994No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG14800.13897405222982928No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG14400.13521799676415822No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC14270.1339972787378151No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA14160.13296436348475557No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA13980.13127413852520362No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC13890.1304290260454276No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA13440.12620346364654766No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA13380.12564005532669703No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG13240.12432543591371213No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG12780.12000597212819043No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC12730.11953646519498153No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA12630.11859745132856378No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG12230.11484139586289271No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA11780.11061583346401276No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG11680.109676819597595No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC11580.10873780573117722No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG11550.1084561015712519No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA11540.10836220018461012No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA11270.10582686274528216No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA11190.10507565165214795No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT11110.10432444055901373No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA11030.10357322946587952No Hit
GGCAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGC10990.1031976239193124No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG10740.100850089253268No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT10690.10038058232005913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG100.007084488145.04407145
CAGGTAC10400.0115.015773
GGTACTG10700.0111.791025
GCAGGTA10900.0110.404912
AGCGAAA1800.0108.75751
AGTAGAA11550.0100.439831
GAGCGAA406.115224E-790.6312561
AGGTACT14850.082.502494
TTATAGG5350.079.9587861
GCGAAAG2800.075.0838852
GACAGTA10150.069.280747
GTAGAAA16900.069.063082
TACTGAT18650.066.469757
CGAAAGC3100.065.4791643
ACTGATC19550.062.668138
GTACTGA20000.061.9830476
AAATATA9700.061.2842525
AGCGAGC753.7535574E-758.017628145
TATGATC1301.8189894E-1255.765226
TAGAAAC22000.055.3596573
Produced by FastQC (version 0.11.5)