FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_F08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_F08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555985
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTATGGCTGCTGTGA57721.0381575042492155No Hit
GTCCAGAGGTCATCCCCCAGCCCTCACCTTGCCAGACTCAGCCTCTTTGA42490.7642292507891401No Hit
GTATCTGACCAACCAGAAAAACTCCAATAGCAAAAACGATCGAAGGAACC41530.7469625979118142No Hit
TGTAGATACTGAGTAGGCAACCGGAAATTCTAGTCTGGAAGTCTGGGCTG37800.6798744570447044No Hit
GTGCTACTGCAGGTCTTTTGTAAAAATGAAATGGCATAACTTGAATTTTC33540.603253684901571No Hit
GAGTGGGGACCTGTATTTCCAATTCTTCTCCTCCCTTCTCTCCTCCCAAT31170.5606266356106729No Hit
ATATTATTCGTGGCTGGGATCGGTATCTGACCAACCAGAAAAACTCCAAT29830.5365252659694056No Hit
GTCATTAACAGAGATGGGAAAGCTGTGGGAGAAACAGGGGTTCAGGGAGA27110.48760308281698245No Hit
CCTAATATACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTATGGCTG26050.4685378202649352No Hit
GAGTAGGCAACCGGAAATTCTAGTCTGGAAGTCTGGGCTGGAGATAAGGT25550.45954477189132803No Hit
CCTAATATCAGTATGAAAGGCAAGACAGAAAGAAACCTAGTGAAACAACA24640.44317742385136294No Hit
GGTTTGGGGTGCCGCGTAGATTTCCAAATGTAGATACTGAGTAGGCAACC23970.43112673903072923No Hit
CTATTTGACAGATTCAGGGTAATGTCCTCTTAAGAAAATAAAATTCTTCA23220.41763716647031845No Hit
GCATTAAAAGATGGTATTCAAAGTCTTGAGACAGTCTGAGATCCTGTAGG23080.41511911292570847No Hit
ACCTAATATACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTATGGCT22720.40864411809671125No Hit
GAGTAGGAGTGACGTGGAAGCAGGCATCGGAGCAGGTGCAGGAGGTGGGA22050.39659343327607766No Hit
GATGTATGGCAATATTATTCGTGGCTGGGATCGGTATCTGACCAACCAGA21970.3951545455363004No Hit
CTAATAATCAGCTCGTGGTTTTTTGTTTAACTTCAGATCAATCCTCCATC21380.38454274845544395No Hit
GTCTGAGATCCTGTAGGCAGTGAGGGTAGAGAGAGAAGAGCCGATGGCCT20480.368355261382951No Hit
CTTTGATACAGTTGCTCATTCCTCCGCCCAGAAGCTCCTTCTGCCCCAGG20420.3672760955781181No Hit
ATTCAGGGTAATGTCCTCTTAAGAAAATAAAATTCTTCAATCAGAACATG19810.35630457656231734No Hit
GATCTATGCCTTTGAAGGAAGCTACCTGGAAGACACTCAGATGTATGGCA19500.35072888657068085No Hit
CCTGTAGGCAGTGAGGGTAGAGAGAGAAGAGCCGATGGCCTCATGGTGCT17260.3104400298569206No Hit
ACCTGGAAGACACTCAGATGTATGGCAATATTATTCGTGGCTGGGATCGG16820.30252614728814625No Hit
CTCTTAATGCTCTCACCAGGTCAGCCATGGCCTCCATGTTGCTAAGTCCA16210.29155462827234546No Hit
GGATGGAGTTGTCATTAACAGAGATGGGAAAGCTGTGGGAGAAACAGGGG16020.28813726989037475No Hit
GTCTTGAGACAGTCTGAGATCCTGTAGGCAGTGAGGGTAGAGAGAGAAGA15920.2863386602156533No Hit
GTATGAAAGGCAAGACAGAAAGAAACCTAGTGAAACAACAAAAACCAACA15530.2793240824842397No Hit
CTTAATGCTCTCACCAGGTCAGCCATGGCCTCCATGTTGCTAAGTCCAGA14160.254683129940556No Hit
GTGTAGGAGGTGGGAACAGGCAGGGGAGCCGGTGTGGGGGCAGGAGTAGG13560.24389147189222732No Hit
GTTCAGGGAGAGAGCAGGTGCTCAGTTTTCTGCATGATAGGTTTGGGGTG13360.24029425254278444No Hit
GAGTGACGGGAAGGATGGAGTTGTCATTAACAGAGATGGGAAAGCTGTGG13160.23669703319334157No Hit
GGGTAATGTCCTCTTAAGAAAATAAAATTCTTCAATCAGAACATGTTGGG12700.2284234286896229No Hit
CACCAGGTCAGCCATGGCCTCCATGTTGCTAAGTCCAGAGGTCATCCCCC12270.22068940708832074No Hit
CTACTGCAGGTCTTTTGTAAAAATGAAATGGCATAACTTGAATTTTCGGC12190.21925051934854356No Hit
GCCCTCACCTTGCCAGACTCAGCCTCTTTGATACAGTTGCTCATTCCTCC12110.21781163160876643No Hit
GTTTTCTGCATGATAGGTTTGGGGTGCCGCGTAGATTTCCAAATGTAGAT12080.21727204870634997No Hit
GGTGTAGGAGGTGGGAACAGGCAGGGGAGCCGGTGTGGGGGCAGGAGTAG11890.21385469032437926No Hit
CTCAGATGTATGGCAATATTATTCGTGGCTGGGATCGGTATCTGACCAAC11840.21295538548701853No Hit
GGTTTAGGGCCATCAGCATTAAAAGATGGTATTCAAAGTCTTGAGACAGT11800.21223594161712994No Hit
CATTAACAGAGATGGGAAAGCTGTGGGAGAAACAGGGGTTCAGGGAGAGA11750.21133663677976922No Hit
GCACTAATGAGTCTTCTAATAATCAGCTCGTGGTTTTTTGTTTAACTTCA11740.2111567758122971No Hit
TCTTAATGCTCTCACCAGGTCAGCCATGGCCTCCATGTTGCTAAGTCCAG11660.20971788807251993No Hit
GCTCTACAGCCTGGAAGCTTCTGCAGCTTCTGCACTAATGAGTCTTCTAA11580.2082790003327428No Hit
GTTGGGAACCATCTGTCAGATCATCCAGCAAAACACGCTGACATCCTTCA11390.20486164195077208No Hit
AGCGAAAGCAGGGCGTTTGGCCTGAGTGACGGGAAGGATGGAGTTGTCAT11240.20216372743868988No Hit
CCTTTGAAGGAAGCTACCTGGAAGACACTCAGATGTATGGCAATATTATT11180.20108456163385702No Hit
GCCTAACCTAATATACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTA11160.20072483969891275No Hit
CTCCCTGCACAGATCCGTCCAGATCCTCAGGGTCCTCCTGGCTGGGCTCA10970.19730748131694198No Hit
AAAGCAGGGCGTTTGGCCTGAGTGACGGGAAGGATGGAGTTGTCATTAAC10720.1928109571301384No Hit
GATTCAGGGTAATGTCCTCTTAAGAAAATAAAATTCTTCAATCAGAACAT10700.1924512351951941No Hit
ATCTTATGCAGTAAATGTTAATGGAGGAGTTGTTCTAGTATCCAGTTTGT10380.1866956842360855No Hit
GTCTTCTAATAATCAGCTCGTGGTTTTTTGTTTAACTTCAGATCAATCCT10340.18597624036619692No Hit
GTATTATAGAGGTCACGGATTGCATGGAGCACTGGGTGTGGCGAAAAAAT10130.1821991600492819No Hit
GCCATGGCCTCCATGTTGCTAAGTCCAGAGGTCATCCCCCAGCCCTCACC9870.17752277489500615No Hit
TCCCAATCCTTCTCCGGTTCTCTCAACCCTCTTAATGCTCTCACCAGGTC9820.17662347005764545No Hit
GCCTGGAAGCTTCTGCAGCTTCTGCACTAATGAGTCTTCTAATAATCAGC9780.17590402618775686No Hit
CCCTTCTCTCCTCCCAATCCTTCTCCGGTTCTCTCAACCCTCTTAATGCT9640.17338597264314684No Hit
GCTCTCACCAGGTCAGCCATGGCCTCCATGTTGCTAAGTCCAGAGGTCAT9530.17140750200095325No Hit
ATCTATGCCTTTGAAGGAAGCTACCTGGAAGACACTCAGATGTATGGCAA9460.17014847522864826No Hit
TGCTACTGCAGGTCTTTTGTAAAAATGAAATGGCATAACTTGAATTTTCG9390.16888944845634324No Hit
GTATTTCCAATTCTTCTCCTCCCTTCTCTCCTCCCAATCCTTCTCCGGTT9010.16205473169240178No Hit
GTTTAAGGAAGCTGAGCGGCTCTTCAGTAAATCCTCAGTTACTTCAGCAG8820.15863737331043104No Hit
TCTCTCAACCCTCTTAATGCTCTCACCAGGTCAGCCATGGCCTCCATGTT8760.15755820750559818No Hit
ACTCAGATGTATGGCAATATTATTCGTGGCTGGGATCGGTATCTGACCAA8710.15665890266823745No Hit
CTGCAGGTCTTTTGTAAAAATGAAATGGCATAACTTGAATTTTCGGCCAG8650.1555797368634046No Hit
GTTTAGGGCCATCAGCATTAAAAGATGGTATTCAAAGTCTTGAGACAGTC8640.15539987589593243No Hit
GGCATAACTTGAATTTTCGGCCAGAGTGGGGACCTGTATTTCCAATTCTT8570.15414084912362744No Hit
CTAATGAGTCTTCTAATAATCAGCTCGTGGTTTTTTGTTTAACTTCAGAT8540.153601266221211No Hit
ATCCTCCATCACGTTTTTCTGTTTTTAAAAAAAACTTCCCAGCCTAACCT8310.1494644639693517No Hit
GCGTTTGGCCTGAGTGACGGGAAGGATGGAGTTGTCATTAACAGAGATGG8310.1494644639693517No Hit
GTGCAGGAGGTGGGAACAGATGCAGGAGCGGGTGTGGGGGCAGGAGTAGG8130.1462269665548531No Hit
GAGTTGTCATTAACAGAGATGGGAAAGCTGTGGGAGAAACAGGGGTTCAG8120.14604710558738096No Hit
TTCCAATTCTTCTCCTCCCTTCTCTCCTCCCAATCCTTCTCCGGTTCTCT8090.14550752268496453No Hit
GTTCTGATGGACCCTATTTGACAGATTCAGGGTAATGTCCTCTTAAGAAA8080.1453276617174924No Hit
GGTCAGCCATGGCCTCCATGTTGCTAAGTCCAGAGGTCATCCCCCAGCCC8070.14514780075002023No Hit
CTCTCAACCCTCTTAATGCTCTCACCAGGTCAGCCATGGCCTCCATGTTG7980.14352905204277094No Hit
ATGCAGGAGCGGGTGTGGGGGCAGGAGTAGGAACAGTCACGGGAACAGGT7980.14352905204277094No Hit
AAGCAGGGCTCTACAGCCTGGAAGCTTCTGCAGCTTCTGCACTAATGAGT7980.14352905204277094No Hit
GCTGTGATGACTCCCTGAGGAAGTAGAAGAAGCAGCCTTCTGAGGCTTCA7960.14316933010782665No Hit
GAACATGTTGGGAACCATCTGTCAGATCATCCAGCAAAACACGCTGACAT7910.14227002527046592No Hit
TGGGAAAGCTGTGGGAGAAACAGGGGTTCAGGGAGAGAGCAGGTGCTCAG7650.13759364011619019No Hit
GAGTAGGAACAGTCACGGGAACAGGTGTGGGAGCAAGTGTGGCCGCAGAG7650.13759364011619019No Hit
AATATACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTATGGCTGCTG7640.13741377914871802No Hit
GTAATGTCCTCTTAAGAAAATAAAATTCTTCAATCAGAACATGTTGGGAA7610.13687419624630162No Hit
CTAATATACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTATGGCTGC7490.13471586463663587No Hit
ATACTGAGTAGGCAACCGGAAATTCTAGTCTGGAAGTCTGGGCTGGAGAT7480.13453600366916374No Hit
GTGGGGACCTGTATTTCCAATTCTTCTCCTCCCTTCTCTCCTCCCAATCC7470.1343561427016916No Hit
GGAGATAAGGTTTAGGGCCATCAGCATTAAAAGATGGTATTCAAAGTCTT7320.13165822818960943No Hit
GAAAGAAACCTAGTGAAACAACAAAAACCAACATTAAAGTCAATTAACTT7260.13057906238477657No Hit
ACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTATGGCTGCTGTGATG7250.1303992014173044No Hit
GTTTGGGGTGCCGCGTAGATTTCCAAATGTAGATACTGAGTAGGCAACCG7250.1303992014173044No Hit
CGTTTGGCCTGAGTGACGGGAAGGATGGAGTTGTCATTAACAGAGATGGG7180.1291401746449994No Hit
CAACTACACCGTGTGTGTGAACCGCCAGCCACTTCTCCAGCTGAAGGAGG7160.12878045271005512No Hit
GCAATATTATTCGTGGCTGGGATCGGTATCTGACCAACCAGAAAAACTCC7150.12860059174258298No Hit
CCTCTTAAGAAAATAAAATTCTTCAATCAGAACATGTTGGGAACCATCTG7150.12860059174258298No Hit
GAGCAGGTGCAGGAGGTGGGAACAGATGCAGGAGCGGGTGTGGGGGCAGG7140.12842073077511085No Hit
CTACAGCCTGGAAGCTTCTGCAGCTTCTGCACTAATGAGTCTTCTAATAA7140.12842073077511085No Hit
AGACAGATCTATGCCTTTGAAGGAAGCTACCTGGAAGACACTCAGATGTA7090.12752142593775012No Hit
CTTCAGATCAATCCTCCATCACGTTTTTCTGTTTTTAAAAAAAACTTCCC7080.127341564970278No Hit
ACTGCAGGTCTTTTGTAAAAATGAAATGGCATAACTTGAATTTTCGGCCA7060.12698184303533366No Hit
AGCGAAAGCAGGTGCTACTGCAGGTCTTTTGTAAAAATGAAATGGCATAA6980.12554295529555654No Hit
GGCGGAAACACTGGCCAACTTGGAGAGACAGATCTATGCCTTTGAAGGAA6950.1250033723931401No Hit
GCATGATAGGTTTGGGGTGCCGCGTAGATTTCCAAATGTAGATACTGAGT6820.12266517981600222No Hit
CCCTGAGGAAGTAGAAGAAGCAGCCTTCTGAGGCTTCACTCCCTGCACAG6780.12194573594611366No Hit
GTTCAGGATCAGCTCATTGAAAAGAGGGAACCAGGAAGTGGGACAGAAAG6730.12104643110875293No Hit
GGTTCAGGGAGAGAGCAGGTGCTCAGTTTTCTGCATGATAGGTTTGGGGT6620.11906796046655935No Hit
GAGCAGGGAGCCCGATGTGGGACTTGATCCCAGGTCCCTGGGATCATTAC6600.11870823853161507No Hit
CCTGTATTTCCAATTCTTCTCCTCCCTTCTCTCCTCCCAATCCTTCTCCG6460.11619018498700505No Hit
GAGATAAGGTTTAGGGCCATCAGCATTAAAAGATGGTATTCAAAGTCTTG6430.1156506020845886No Hit
GGAGAGAGCAGGTGCTCAGTTTTCTGCATGATAGGTTTGGGGTGCCGCGT6420.11547074111711647No Hit
CTGATGGACCCTATTTGACAGATTCAGGGTAATGTCCTCTTAAGAAAATA6330.11385199240986717No Hit
GCACAGATCCGTCCAGATCCTCAGGGTCCTCCTGGCTGGGCTCATTCTCC6280.11295268757250645No Hit
TGGCAATATTATTCGTGGCTGGGATCGGTATCTGACCAACCAGAAAAACT6190.11133393886525716No Hit
GGGCTCTACAGCCTGGAAGCTTCTGCAGCTTCTGCACTAATGAGTCTTCT6140.11043463402789643No Hit
AACTTGAATTTTCGGCCAGAGTGGGGACCTGTATTTCCAATTCTTCTCCT6120.11007491209295214No Hit
CTCACCAGGTCAGCCATGGCCTCCATGTTGCTAAGTCCAGAGGTCATCCC6070.10917560725559143No Hit
GCGAAAGCAGGTGCTACTGCAGGTCTTTTGTAAAAATGAAATGGCATAAC6050.10881588532064715No Hit
GGGAGAGAGCAGGTGCTCAGTTTTCTGCATGATAGGTTTGGGGTGCCGCG6050.10881588532064715No Hit
CTCCCTGAGGAAGTAGAAGAAGCAGCCTTCTGAGGCTTCACTCCCTGCAC6040.10863602435317499No Hit
GATCAATCCTCCATCACGTTTTTCTGTTTTTAAAAAAAACTTCCCAGCCT6000.10791658048328642No Hit
CAGATGTATGGCAATATTATTCGTGGCTGGGATCGGTATCTGACCAACCA5950.10701727564592571No Hit
CTTCTGCACTAATGAGTCTTCTAATAATCAGCTCGTGGTTTTTTGTTTAA5920.10647769274350927No Hit
AAACAGGGGTTCAGGGAGAGAGCAGGTGCTCAGTTTTCTGCATGATAGGT5920.10647769274350927No Hit
GGCCTGAGTGACGGGAAGGATGGAGTTGTCATTAACAGAGATGGGAAAGC5890.10593810984109284No Hit
GTTTTTATTCTTTCGTTTTTTATGGCTGCTGTGATGACTCCCTGAGGAAG5890.10593810984109284No Hit
GCTCTTCAGTAAATCCTCAGTTACTTCAGCAGCTGCAGTAAGTGCGTTGG5860.1053985269386764No Hit
GTGTGGGGGCAGGAGTAGGAGTGACGTGGAAGCAGGCATCGGAGCAGGTG5840.10503880500373211No Hit
AAGCAGGAGCTGGCGGAAACACTGGCCAACTTGGAGAGACAGATCTATGC5800.10431936113384353No Hit
ATGTAGATACTGAGTAGGCAACCGGAAATTCTAGTCTGGAAGTCTGGGCT5770.10377977823142712No Hit
ATGTTGCTAAGTCCAGAGGTCATCCCCCAGCCCTCACCTTGCCAGACTCA5750.10342005629648282No Hit
CTTCTCTCCTCCCAATCCTTCTCCGGTTCTCTCAACCCTCTTAATGCTCT5750.10342005629648282No Hit
ATTTGACAGATTCAGGGTAATGTCCTCTTAAGAAAATAAAATTCTTCAAT5690.10234089049164996No Hit
AATGAGTCTTCTAATAATCAGCTCGTGGTTTTTTGTTTAACTTCAGATCA5670.10198116855670566No Hit
CCTCCCTTCTCTCCTCCCAATCCTTCTCCGGTTCTCTCAACCCTCTTAAT5640.10144158565428925No Hit
AACTTGGAGAGACAGATCTATGCCTTTGAAGGAAGCTACCTGGAAGACAC5620.10108186371934494No Hit
GGTGCTACTGCAGGTCTTTTGTAAAAATGAAATGGCATAACTTGAATTTT5570.10018255888198423No Hit
TTTTATGGCTGCTGTGATGACTCCCTGAGGAAGTAGAAGAAGCAGCCTTC5560.10000269791451209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAACG100.0070806895145.06389145
GGAGTGT100.0070806895145.06389145
CCATTAT100.0070806895145.06389145
AAAGACC100.007090219144.998644
CAAAGAC100.007090219144.998643
AGCGAAA4350.0123.354371
TTGCCAC950.0122.15906145
GCGCACC850.093.86487145
TCTATGT406.1142055E-790.624153
AAACAAG259.378182E-487.014851
AGGGTGT259.3848875E-486.999196
ACAAGGG259.3848875E-486.999193
AACAAGG300.001934669272.505852
CAAGGGT300.001935359872.499334
TATGTTG502.3050197E-672.499335
CGAAAGC7500.071.532673
CCCTGGA851.6916601E-1068.24082
GGTGTAG2450.065.1131441
CCTGGCC451.1907948E-464.47284145
GCTCCGG350.003559453262.170235145
Produced by FastQC (version 0.11.5)