FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837334
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAATACAGGTCAAAAATGTAAAAAGGTTATAAACCTTAACAGGAAAA77050.9201823883898181No Hit
GCCCAGGGCAGCCCTGTCCTCCAGGCAATGGGAGAGTGTGAGCGGATGCA35820.4277862836096468No Hit
CGTCAGCACCTTTCTGTGGCCAGGCTTGGGGAAGGAGTACGATTTGATTC32300.38574810051902825No Hit
GGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAAGA26870.3208994260354888No Hit
GTTATAAACCTTAACAGGAAAAATAAAACAACTTTTACTGGCCATTCCTG24920.2976112280165382No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC21410.2556924715824271No Hit
GCATAAAGCCAAGTAACAAATTGCTACCTTCAACAAGCAGAGCTAGACTC20800.24840744553547328No Hit
GATCAGGTCATACCAAAATAAAAACAGGAAAAGAAAAAGTTAGAACACGA20600.24601891240532453No Hit
GAATACATACAGTAGGTAGGCTAAAACAGAAGAGTCTTTAGAACTAGTGC18550.2215364478212995No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG18280.21831192809559866No Hit
CCCCCAAAATGCATAAAGCCAAGTAACAAATTGCTACCTTCAACAAGCAG17540.20947435551404814No Hit
GTATAACACGGGGACGGAGGTGGGATACCAGCACTTCTCCAGCATGGGCA16910.2019504761540795No Hit
TCTCCAGCATGGGCAAGTAGGCAGTTGCTGACGGCGGGATCAGATAGAAG16130.19263519694649925No Hit
GTGCAGAGACTGTCCCTGTGCTGTGGGTCCCTGTGCTGGTGGCCTGACCG16030.19144093038142487No Hit
GGCTAAGGCCACGAGGGTACGGGATCACAGAGCCTGTGTGTCTTGCTCTG15980.19084379709888766No Hit
GGGCAGCCCTGTCCTCCAGGCAATGGGAGAGTGTGAGCGGATGCAGTCGG15930.19024666381635047No Hit
GAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATAAAGCCA15920.19012723715984303No Hit
GGCTTGGGGAAGGAGTACGATTTGATTCTGGAAATTATTCCTCCCGCCGA15790.18857469062524632No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG15670.18714157074715704No Hit
GGTCATACCAAAATAAAAACAGGAAAAGAAAAAGTTAGAACACGATCGGC15580.1860667308385901No Hit
GAACTGTGAGAACTCTCAGCAGTCCATCGGGTTTGTAAGAGTAATGCTCC15240.18200622451733717No Hit
CTTTTGGACTAAGACAAAAACATTGCCATTACTGTTGAAATTTTGCATTT14980.17890113144814374No Hit
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA14790.17663202497450242No Hit
GGGCAGAGGAGTCGATAGCTGGATGGGAAGGCAAGGGAGAAGGGAGTCTC14620.17460177181387596No Hit
ATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCT14510.17328807859229411No Hit
GAGCTAGACTCGGTTTGCAGTTCTATCCTAAAACTCCTTTTCACCAAGCT14440.17245209199674205No Hit
GTTCCAGTCACCCCCAAAATGCATAAAGCCAAGTAACAAATTGCTACCTT14310.17089954546214534No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA14310.17089954546214534No Hit
ATAATAATCAAGGGTTACAGAAAGAGACTTTATCTCAGATTGAATACATA14300.1707801188056379No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG14250.1701829855231007No Hit
GGTCTGAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATAA14010.16731674576692218No Hit
GGTATAACACGGGGACGGAGGTGGGATACCAGCACTTCTCCAGCATGGGC13680.16337566610217666No Hit
GAGTACGATTTGATTCTGGAAATTATTCCTCCCGCCGACCACGTGGCAGG13520.16146483959805766No Hit
GTCCCTGTGCTGTGGGTCCCTGTGCTGGTGGCCTGACCGTGCAGCCTCTC13410.16015114637647582No Hit
GCCCCACACCACGCTCTGGCAGCCAGTGGAGCCGTAGTGGCTGGATTGTG12820.15310497364253692No Hit
GCAGGGGACACGTCAGCACCTTTCTGTGGCCAGGCTTGGGGAAGGAGTAC12700.15167185376444764No Hit
AAAGCAGGGTCTGAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAA12270.14653650753462777No Hit
ATCAAGCATAGTGCATTTTGAAAAGCTGGTGCAGGGCTAGCTGGACGAGT12260.1464170808781203No Hit
GCTTAAAGGCCTAACCAGCTCTTGGCACAGCAAGATCCTTTCTGCAGGCT12220.14593937425209058No Hit
TTCCTGAGTAGAGTGCTTACCAAATGCCCCACACCACGCTCTGGCAGCCA12180.1454616676260608No Hit
TTTGTACCCCGTCTTGCTTTTCTTATCTTTGGTGCCCAATAATAATCAAG12050.1439091210914641No Hit
GGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCA11820.141162307991793No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT11810.14104288133528556No Hit
GGTTATAAACCTTAACAGGAAAAATAAAACAACTTTTACTGGCCATTCCT11390.13602696176197313No Hit
ATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCA11210.13387728194483922No Hit
TTGCAGTTCTATCCTAAAACTCCTTTTCACCAAGCTTAGCTTAAAGGCCT11170.13339957531880944No Hit
CCCGGGAGGGGCGCGCGGGCTCCGAAGTTGATGTTTCCTGTCTGCCCGCC11160.13328014866230203No Hit
GACAAAAACATTGCCATTACTGTTGAAATTTTGCATTTTTGTACCCCGTC10850.12957792231057141No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG10770.1286225090585119No Hit
GGGATGGACAGCTTGCCCGTCTTGTCTTTGTCGATCCGGTAGTGAAGCAC10770.1286225090585119No Hit
AAGCAGGGCAGAGGAGTCGATAGCTGGATGGGAAGGCAAGGGAGAAGGGA10740.12826422908898957No Hit
GCTTAGCTTAAAGGCCTAACCAGCTCTTGGCACAGCAAGATCCTTTCTGC10580.12635340258487054No Hit
ATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTT10580.12635340258487054No Hit
AATTACCACATCGTGGACGTCAAGGGTGAAAACATCACCAATGCCCCTGA10520.12563684264582592No Hit
GGCCTAACCAGCTCTTGGCACAGCAAGATCCTTTCTGCAGGCTAATGCCC10440.1246814293937664No Hit
ATCCTAAAACTCCTTTTCACCAAGCTTAGCTTAAAGGCCTAACCAGCTCT10330.12336773617218456No Hit
GCTTTATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGT10240.12229289626361761No Hit
GTAACAAATTGCTACCTTCAACAAGCAGAGCTAGACTCGGTTTGCAGTTC10240.12229289626361761No Hit
GTGCTGAAGAAGTACGAGCTCCGGGTTAATTACCACATCGTGGACGTCAA10230.12217346960711019No Hit
GAGTAATGCTCCACCAGCTGCCAGAGCGTGTCGAACTTCTTCCCGTCGGG10190.12169576298108042No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA9990.11930722985093166No Hit
GTTCTATCCTAAAACTCCTTTTCACCAAGCTTAGCTTAAAGGCCTAACCA9920.11847124325537958No Hit
GCATATGCCTGACCCTTCTGATGCCAGAAAGCCATGGCCCTTGGTGTGTG9700.1158438568122159No Hit
GTATATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGCCAATCG9630.11500787021666384No Hit
ACTCCATCCTGTGTGCAGAGCCCGACCTGGGGCTCAGTCCCACAACTCTG9620.11488844356015639No Hit
GTAAAAAGGTTATAAACCTTAACAGGAAAAATAAAACAACTTTTACTGGC9580.11441073693412665No Hit
ACTCTCAGCAGTCCATCGGGTTTGTAAGAGTAATGCTCCACCAGCTGCCA9360.11178335049096298No Hit
TACCAGCACTTCTCCAGCATGGGCAAGTAGGCAGTTGCTGACGGCGGGAT9310.11118621720842578No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA9290.11094736389541092No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC9210.1099919506433514No Hit
CCTTAACAGGAAAAATAAAACAACTTTTACTGGCCATTCCTGTTGAAATG9190.10975309733033652No Hit
TTACCACATCGTGGACGTCAAGGGTGAAAACATCACCAATGCCCCTGAGC9170.10951424401732164No Hit
GGATGGCAGGGGCCTCGGGCTAAGGCCACGAGGGTACGGGATCACAGAGC9070.10831997745224727No Hit
GGACACGTCAGCACCTTTCTGTGGCCAGGCTTGGGGAAGGAGTACGATTT9070.10831997745224727No Hit
TAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAG9050.10808112413923238No Hit
CCTTTCTGTGGCCAGGCTTGGGGAAGGAGTACGATTTGATTCTGGAAATT9000.10748399085669519No Hit
GAGCAAGGGGGCTGAGATCTTGTCTGGGTTCATGAAACTGGTGAGGGCTG8930.1066480042611431No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC8900.10628972429162079No Hit
GTCCATCGGGTTTGTAAGAGTAATGCTCCACCAGCTGCCAGAGCGTGTCG8880.10605087097860591No Hit
GAGGTGGGATACCAGCACTTCTCCAGCATGGGCAAGTAGGCAGTTGCTGA8820.10533431103956128No Hit
GGTCAAAAATGTAAAAAGGTTATAAACCTTAACAGGAAAAATAAAACAAC8720.10414004447448688No Hit
GGAGAAGGGAGTCTCTGGGGCATGAGCAAGGGGGCTGAGATCTTGTCTGG8670.1035429111919497No Hit
GGTCAGGAAGGCCGCTGCTACCTGCGGGGTGCGAACATGATGCGTGGTGT8600.10270692459639762No Hit
TCAACAAGCAGAGCTAGACTCGGTTTGCAGTTCTATCCTAAAACTCCTTT8600.10270692459639762No Hit
GGGTCTGAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATA8570.10234864462687529No Hit
GTGTGAGCGGATGCAGTCGGGCCCTGGGGAGCAGGGCTGCGGTCCGTGGT8560.10222921797036785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA950.0122.145051
AGCGAAA5850.0121.492571
AGTAGAA3250.0104.880321
GGTGCGC1100.092.286255145
GCGAAAG8300.086.473092
GGTACTG3950.086.263015
CTAATAC13950.083.671113
GGATAAC801.8189894E-1281.5598456
TAATACA14800.080.335224
GGACTAA4600.077.225756
ATACAGG15450.075.547716
TCTAATA16000.073.403862
TGGACTA4850.073.245045
TTGGACT5100.071.0761264
CGAAAGC10200.070.3653643
ACTGATC5150.067.570618
TACTGAT5150.066.1628957
GCTACCG1009.094947E-1265.25957145
ATAACCT1009.094947E-1265.247888
CTACTAG350.003566761362.1408356
Produced by FastQC (version 0.11.5)