FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114666
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT38550.3458435082796102No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA23730.21288888330674838No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG20280.1819379078576004No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA18440.16543072095138814No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA14440.12954553202483973No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT14080.12631586502145037No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC12980.11644743806664958No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC12770.11456346564800578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3400.055.462611
ATAGACC18050.036.9522673
TATAGAC19200.035.495772
TAGACCT20650.033.7040184
CGAAAGC6000.032.6244353
CAGCTCG12100.030.5559489
TAGGTAC18850.030.396362145
AAGGTAT8600.030.3483126
GTATATA24400.029.1301081
CCTCTTA22600.028.8698888
GCGAAAG6550.028.7897531
ATAGTAC4050.028.641483
TCGATAA1050.007711164727.617339
TATACAG13750.027.4177075
AGACCTC25550.027.2402325
CGTAGAC1100.00967286926.363183
TTACGCA2808.200004E-925.892414
GTATTAG3709.094947E-1225.4828191
ATATATG12350.025.2424773
GAGCGAA2301.0995955E-625.2271391
Produced by FastQC (version 0.11.5)