FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122613
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT11500.10243957623865035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3850.088.5272141
TTACGTC350.003564568562.15187145
CGAAAGC5800.060.0006833
GCGAAAG7200.048.333882
GGGCTAT1801.2899909E-732.2297631
GTATAGG3000.031.424021
TTTGACG1402.7467593E-531.0717834
GCGTTGT1255.1552383E-428.9990399
GTATTAC6800.028.7935051
TAGGTAC10500.028.313627145
TATAGAC12050.027.0749972
CACGATG2001.0644229E-525.37868145
GCTATAC1751.255763E-424.8574263
CTATACT4450.024.438484
GTATACT1500.001494288124.166943
TACATCA7200.024.1658655
ACCGTCG1500.001494675824.1658658
TAGGATA2802.3428947E-723.3038374
GTACCAG1902.1899267E-422.9000971
CGTAAGA1952.6148197E-422.3079452
Produced by FastQC (version 0.11.5)