FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_D06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_D06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311139
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG21320.16260671065386662No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA16650.12698882422077293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3800.055.34351
GCGAAAG6900.033.6217732
CGAAAGC7250.032.998613
TATACAG12900.031.4715445
CAGCTCG12050.029.4800879
ATATACT13050.028.332144
CTAGACG1050.007710893627.6178873
TATATAC26850.027.5407413
TATATGT12100.023.9659334
TATACTT13500.023.6286375
ATATATG11250.023.1990223
GTATATA23550.023.0952451
TATACTG8550.022.8937745
ATACAGC16500.022.8475236
TACTCCG2252.6532174E-522.5546075
CTATAGT3554.129106E-922.4638424
ATACTTA14050.022.1876776
ATACCGA2353.7115184E-521.5948356
TACTTAC13900.021.3839937
GCGTTAG1750.00365375620.7197341
Produced by FastQC (version 0.11.5)