FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822424
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT81360.9892707411262316No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA43410.527829927142204No Hit
TCTATATTCTATACGTGTAAAGCTCACACTATGTTGTTAGGCAAATTTAT41810.5083752419676468No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC39350.4784636635117652No Hit
CCTCTAGAGAGGTAATATCGAAGCAGAAATCTGAATGGCATGAGGCATCC34530.41985642442341176No Hit
GTTTAAGACCAGCATCCAAGCCAGTGTACCTAGAGTCAGGGACCTGGGCT22780.2769860801727576No Hit
CCTTGGAGAAGGACCTGCATGGTGGGGGTGATATTGGGAAGGGAGACACG21930.2666507786737741No Hit
GTCTTAATGCAGCTTACATAATGCAGATAGCTGTTAGTTTAACAGCTACC21520.26166551559779383No Hit
CATCTACATCTATATTCTATACGTGTAAAGCTCACACTATGTTGTTAGGC20120.2446426660700563No Hit
GTCCCAGGACCCTGGGATCATGACCTGAGCTGAAGGCTTAACCGACTGAG18930.23017324397147945No Hit
AAAGCAGGGAGCCCAAAGGGTAGTTTATATGAGAAAGGAGGGGAATGAAA18660.2268902658482729No Hit
GCCCAAAGGGTAGTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAG18640.22664708228359093No Hit
GTTTATTCCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATT18310.22263455346633854No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT18050.219473167125473No Hit
GTGTAAACACTCAAGGGCAGCAGGCTGTTGGTTAAAATACTGAGTCTTAA17310.2104753752322403No Hit
GAATATAGATGTAGATGTATGTGTAGAATACAGAATACGTGTAGAAATAA17300.21035378344989933No Hit
GATCTGAGCAGCGAATGATGTGATCTATTTCTCAGTGTTTTTACCCTTTG16270.19782982986877815No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA16110.19588436135132242No Hit
GGCTGGAGCTGACCACGTGGTGGAAAGAGCCAGTTTGGGAACTAGGTGTC15760.19162864896938805No Hit
CCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATTGCTCCAG15520.18871044619320448No Hit
GGGTAGTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAA15300.1860354269817029No Hit
TAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGT15080.18336040777020127No Hit
GTCTTATAGGGGTTCAGAGAAAATACCTACAAATCATACACATGAGCCAA15020.18263085707615537No Hit
ACATAATGCAGATAGCTGTTAGTTTAACAGCTACCCTGTGCTCACCAACG14950.1817797145997685No Hit
GGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA14720.1789831036059259No Hit
GAAAAGGGAGATCCCATCTTGTCACACCTCTTCCCGTGGGGCAGGCGAGG14680.17849673647656197No Hit
GCATGGTGGGGGTGATATTGGGAAGGGAGACACGTCAGATCCCCAGAGAG14450.17570012548271938No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC14440.1755785337003784No Hit
ACCTTAGACAGCAGCCACATGGGTTCCTCCCAAGTCGTGCATAGGAGAAT14270.17351147340058168No Hit
GGTTTAGGGGGGACATCCAGCCCAGGTCAGGCAGGCTGGGAGAATGCTGG14220.1729035144888768No Hit
GTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATTGCTCCAGGTCCA14170.17229555557717188No Hit
ATATTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGACA14100.171444413100785No Hit
ATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGTTTGTG13550.16475686507203097No Hit
GTATAGATTTTTTAATTCTTTGGTTATGTTTATTCCTAGGTACTTGCCAT13500.16414890616032607No Hit
GGCTGGGAGAATGCTGGCTGTACGGAAAGGCTGGAGCTGACCACGTGGTG13490.16402731437798507No Hit
GTTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTAC13340.16220343764287035No Hit
TTCTATACGTGTAAAGCTCACACTATGTTGTTAGGCAAATTTATGTCCTG13310.1618386622958474No Hit
GTGTATAGATTTTTTAATTCTTTGGTTATGTTTATTCCTAGGTACTTGCC12990.15794772526093595No Hit
ATCCAGCCCAGGTCAGGCAGGCTGGGAGAATGCTGGCTGTACGGAAAGGC12940.15733976634923105No Hit
CCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCG12910.1569749910022081No Hit
GTATTCTACACATACATCTACATCTATATTCTATACGTGTAAAGCTCACA12770.15527270604943436No Hit
CAGCTACCCTGTGCTCACCAACGCCCTGGCAGCCCTCAGCCCTGCCACTT12700.15442156357304748No Hit
GTGCAAGGCACCCAGCCTCGGAGTAGCTGTGTCCCCTTCCGAGGGACTGC12670.15405678822602453No Hit
ATCCATGAGCATGGATTGTCTTCCCGTTTGTTTGTGCCATCTTCAATTTC12410.150895401885159No Hit
ATTCCAGACAGAGGAAAGGGCGTGAGCTTGGTATGTTTAAGACCAGCATC12300.1495578922794082No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA12240.1488283415853623No Hit
GTTTTTACCCTTTGTGTAAACACTCAAGGGCAGCAGGCTGTTGGTTAAAA12230.14870674980302132No Hit
GCACTGGAACATGTTAGCAACACCAGCCGTGGGCTCGGGAGCCCAGAGTG12110.14724764841492952No Hit
TATATACACTATCTCCTTTGATCATCACACCAATCCATAAAGAAAGGGCA12060.1466396895032246No Hit
GTGTAAAGCTCACACTATGTTGTTAGGCAAATTTATGTCCTGGTACTCTC11540.14031691682149353No Hit
GACATAAGCTGGAATGTGAGGATTTCTGAAACGCGTGGCACTGGAACATG11530.14019532503915255No Hit
CTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTT11510.1399521414744706No Hit
TGTCACACCTCTTCCCGTGGGGCAGGCGAGGCAGGAGACATAAGCTGGAA11280.137155530480628No Hit
CTATGTTGTTAGGCAAATTTATGTCCTGGTACTCTCTGTTTCTTTGTAGA11250.13679075513360503No Hit
GATCTGGACCAAGCCAAGGAGGAGGTGTTCCGCCTACCCCAAGAGGAGGA11140.13545324552785423No Hit
TGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACCGTGGTGA11020.13399414413976246No Hit
GAGCAGGGAGCCTCAGTCCCAGGACCCTGGGATCATGACCTGAGCTGAAG10910.13265663453401164No Hit
GTGTTACAGTTGCCCTAAGTTTTATTTCTACACGTATTCTGTATTCTACA10890.1324134509693297No Hit
CCCACATTCTGACCTTAGACAGCAGCCACATGGGTTCCTCCCAAGTCGTG10890.1324134509693297No Hit
ATAGTAGGGAGAGCACCAGGCATTAGAGGTCATAAGGCCCCTAATGTGGT10820.13156230849294281No Hit
CTCAAGGGCAGCAGGCTGTTGGTTAAAATACTGAGTCTTAATGCAGCTTA10800.13131912492826084No Hit
GGTCAAGAAAGAAAAGGGAGATCCCATCTTGTCACACCTCTTCCCGTGGG10720.130346390669533No Hit
ATGTAGATGTATGTGTAGAATACAGAATACGTGTAGAAATAAAACTTAGG10590.12876569749910022No Hit
GGGTAGTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGG10580.12864410571675924No Hit
CATCTATATTCTATACGTGTAAAGCTCACACTATGTTGTTAGGCAAATTT10430.12682022898164452No Hit
ATGTTGTTAGGCAAATTTATGTCCTGGTACTCTCTGTTTCTTTGTAGAAA10370.1260906782875986No Hit
AGCATGGATTGTCTTCCCGTTTGTTTGTGCCATCTTCAATTTCTTTCATC10310.12536112759355272No Hit
GTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGG10090.12268610838205112No Hit
TTCTAGTTCTGTGAGAAGTGTTATTGGTATTTTGATAGGTATTACATCAA10020.12183496590566423No Hit
GTATAGGAAGGAACCGACTTTCCCATATCTAAGGTCACACAGAGAGAGGA9990.12147019055864128No Hit
TTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTACA9910.12049745629991342No Hit
CTCATGACCCAGCCGCACAGAAGCCGAACCACAAAGCAGAGCCCAAAAAT9790.11903835491182163No Hit
ATCAAAACACCGTGGTGAACTCCTTGGAGAAGGACCTGCATGGTGGGGGT9790.11903835491182163No Hit
GAGGAAAGGGCGTGAGCTTGGTATGTTTAAGACCAGCATCCAAGCCAGTG9650.11733606995904788No Hit
CTAGAGAGGTAATATCGAAGCAGAAATCTGAATGGCATGAGGCATCCACT9530.1158769685709561No Hit
CCACTGAGCAGGGAGCCTCAGTCCCAGGACCCTGGGATCATGACCTGAGC9520.11575537678861511No Hit
GTTTAGGGGGGACATCCAGCCCAGGTCAGGCAGGCTGGGAGAATGCTGGC9460.11502582609456923No Hit
GAATGAAAGAGAAAATAGAATCAAAACACCGTGGTGAACTCCTTGGAGAA9430.11466105074754629No Hit
TCCCCAGAGAGGTTTAGGGGGGACATCCAGCCCAGGTCAGGCAGGCTGGG9370.11393150005350038No Hit
CTCCCAAGTCGTGCATAGGAGAATCTCTTCCCTCCGGCATCCTCTTGATT9350.11368831648881843No Hit
CGTCAGATCCCCAGAGAGGTTTAGGGGGGACATCCAGCCCAGGTCAGGCA9200.11186443975370369No Hit
GGCATGAGGCATCCACTCAAAGATCTGGGGAAAGAACATTCCAGACAGAG9170.11149966440668073No Hit
CCTCTTCCCGTGGGGCAGGCGAGGCAGGAGACATAAGCTGGAATGTGAGG9100.11064852193029386No Hit
GTAGAATACAGAATACGTGTAGAAATAAAACTTAGGGCAACTGTAACACA9070.11028374658327092No Hit
CCCAGGCACCCCTCGCTGAGTATTTTTTAGGATTCCATTGGCTCTCCATC9070.11028374658327092No Hit
TTTTAATTCTTTGGTTATGTTTATTCCTAGGTACTTGCCATCCCCTCTCC9010.10955419588922503No Hit
ACCTAGAGTCAGGGACCTGGGCTTCGGGTGGAGGAGACCCAGACAGGTGG8790.10687917667772341No Hit
AGAAAAGGGAGATCCCATCTTGTCACACCTCTTCCCGTGGGGCAGGCGAG8770.10663599311304145No Hit
ATCCAAGCCAGTGTACCTAGAGTCAGGGACCTGGGCTTCGGGTGGAGGAG8620.10481211637792673No Hit
CTTCAATTTCTTTCATCAGTGTTTTCTAGTTTTCAGTGTATAGATTTTTT8590.10444734103090376No Hit
GGGAAGGACTGGAAAAGATCAGAGGAGAGAGACAGGGAATGGGCCTGAAG8560.10408256568388081No Hit
GCATCATTGATTCTCATGACCCAGCCGCACAGAAGCCGAACCACAAAGCA8550.10396097390153983No Hit
GGTAATATCGAAGCAGAAATCTGAATGGCATGAGGCATCCACTCAAAGAT8520.10359619855451689No Hit
TCTATACACTGAAAACTAGAAAACACTGATGAAAGAAATTGAAGATGGCA8480.10310983142515295No Hit
GCTCACACTATGTTGTTAGGCAAATTTATGTCCTGGTACTCTCTGTTTCT8420.10238028073110707No Hit
GTATATTTCCATTTCCTCCCTTTCTTGTCCTTTGTGTTACAGTTGCCCTA8410.10225868894876608No Hit
TGTTTATTCCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAAT8410.10225868894876608No Hit
CTTTTGTGGTTCCATACAAATTTTAGGATTATTTGTTCTAGTTCTGTGAG8380.10189391360174314No Hit
TTCTAGTGGTTGCTCTCTGGTTTACAGGTGGAACTCATCACAGGCTGCCG8370.10177232181940216No Hit
TCCTGGTACTCTCTGTTTCTTTGTAGAAAGGGCCTTTTAAAATCGAAGGA8370.10177232181940216No Hit
GTTAAAATACTGAGTCTTAATGCAGCTTACATAATGCAGATAGCTGTTAG8290.1007995875606743No Hit
AATCAAAACACCGTGGTGAACTCCTTGGAGAAGGACCTGCATGGTGGGGG8240.10019162864896938No Hit
TTTATGTCCTGGTACTCTCTGTTTCTTTGTAGAAAGGGCCTTTTAAAATC8230.10007003686662841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5000.0127.642231
GAGCGAA256.4535598E-6116.038381
TAGGTAA900.096.6869145
TCTGCCG259.378351E-487.01821145
TCTCGTT300.001934013272.515175145
AGTGACT300.001935880372.497549
CGAAAGC9400.067.874163
CGCTTGG554.0559207E-665.92288145
TCTAGAG7000.064.216013
AAAAGGT2300.063.0413365
GGGGCGT350.003566758762.1407436
ATAGGGA753.7596874E-758.0015563
ATGTAGT1051.1059456E-955.256371
CGTGGTT400.006045498454.38638145
TATGGAC805.8639307E-754.38638145
GGACCAA4000.054.3731586
TGGACCA4050.053.7018745
TCGAACT450.00963056748.34345145
TGTAGTG1203.616151E-948.337572
TACATCA16850.047.327775
Produced by FastQC (version 0.11.5)