FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674398
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACATGCTCTTCAGGGTAACTAACTGGCCAGCCACGTTCCTCCA16050.2379900296264224No Hit
GGTATGAGAAGAGCCTTTGTTCTCGTGGAGGTGGTGTGGGACGAGGCAAA14660.21737905509802816No Hit
GGACAGGCGAGAGCGGGCAACTCGTCAAGCGAGCAGCCTTCGGAGAAGGG13130.1946921550775655No Hit
GTGCTAATCTGTGAACTCCGTCTCCCGTCTCCCGACTAGAGTGCGGCCAC12570.18638845310929153No Hit
TCTCATACCTGTCTCAGTTCTCTCAGTTCACCCCTTCGCCCTGAATGTCC12510.1854987707555479No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT12380.18357112565577002No Hit
ATCTAATACAGGTCAAAAATGTAAAAAGGTTATAAACCTTAACAGGAAAA12200.18090207859453913No Hit
GTATAACCCCAGATCTGTTCATAAGGGCCAGATATGAATAAGAGGGCCAC10390.15406332758993946No Hit
GTGCAGAGCCGGGCAAACGTGGGGGATTCACATGCTCGCCATGAACCTGG9660.14323885895272526No Hit
CTTCAGGGTAACTAACTGGCCAGCCACGTTCCTCCACGACAGGGAGGAGG9600.1423491765989816No Hit
ATCCTAAACAGAGTCAGTGCAGAGCCGGGCAAACGTGGGGGATTCACATG8430.12500037070098072No Hit
GAATAAGAGGGCCACCTCTGTCTCTTGAGGTCATTTCCAGAGAAAGCTTG7770.11521386480980074No Hit
GAATGTGTGTGGCTCCCACAGGGGCAGCCGGACAGGGGAGGCAGGTGGCA7710.1143241824560571No Hit
GTCACAGGGACAGCGGTCACACACTGGCAGGGAAGAGAAAGCAGATGAGC7630.11313793931773226No Hit
GTACCAGGACTGGGAAGATCGAATGCGGGGATCTGTAAGTCACAGTGACT7270.10779984519527046No Hit
CCCCAGATCTGTTCATAAGGGCCAGATATGAATAAGAGGGCCACCTCTGT7240.10735500401839863No Hit
ATCTCATGCTGGGTTTTTGTTCTTTCTGTTTTAAAATAAGACCTAGAAGG7030.1042411157802959No Hit
GTGTATACTCCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAG6870.10186862950364621No Hit
GTGTGTGCACAGCTGCGCGGTCAGCCCATCCTAAACAGAGTCAGTGCAGA6860.1017203491113556No Hit
CTATGTGTACTGCATAACAAACCGCTCCCACCTAACTAAGGAATTTCAAA6810.10097894714990259No Hit
GTTCTAGGTGGCCTAAGCCAGGAAAACTAAGGGAACACATCGCTCGGGGT6800.10083066675761196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACT100.0070896167145.005044
AGCGAAA2400.0102.765251
TAATACA3050.068.936824
AGACGAT451.1933722E-464.4466866
ATCGATA350.00356550962.1450166
AATCGAT350.00356550962.1450165
TAATCGA350.00356550962.1450164
CGATATT350.003566556662.1404088
GCGAAAG4150.061.1512372
CGAAAGC4100.060.1240433
CTAATAC3850.058.378653
CGATGTA502.0101636E-457.997729
TAACGCG651.0930353E-555.771175
TATAGAC3450.054.6436352
AACGCGA701.6950426E-551.7875146
TTAACGC701.6950426E-551.7875144
TGAGACG701.6950426E-551.7875144
ACGACCT450.00963646348.3350145
AATGATG803.732585E-545.3140756
AGGCGTA2100.044.882517
Produced by FastQC (version 0.11.5)