FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107897
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT32960.2975005799275564No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA27060.24424653194295137No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC26640.24045556581523375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC400.006048836354.380047145
TATACCG400.006052077754.3726845
AGCGAAA3600.048.3465461
GCGAAAG5750.034.0420272
GTCTAAG5150.032.387491
GGATAGC4950.032.2208485
GGCGGTT1352.1430333E-532.2208487
CGAAAGC6000.030.2070453
GTATTAC14400.029.712981
TAGACAG6350.029.6837735
CAATTCG3201.8189894E-1229.451879
GATAGCA6000.026.58226
GGTACTG6050.026.3625135
TATTACA17500.026.0988862
CTTAGAC4450.026.0663053
GTATTAG3108.56744E-1025.7328381
TACATCA16550.025.406775
ACATCAA16450.025.120516
TTACATC16550.024.5306764
CCTCGCG1500.001493567724.168907145
Produced by FastQC (version 0.11.5)