FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_B03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_B03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369099
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC21760.5895437267508176No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT12290.33297299640475864No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT10460.2833928024730492No Hit
CTTAACGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAA9810.26578235107654047No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA9280.25142305993784864No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA9200.24925561976597066No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC7110.19263124527565775No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC6830.18504520467408472No Hit
GCTTAACGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATA5780.1565975524181859No Hit
GATATTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC4360.1181254893673513No Hit
GGTTTAGGGGGGACATCCAGCCCAGGTCAGGCAGGCTGGGAGAATGCTGG4140.11216502889468678No Hit
GTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGG4130.11189409887320204No Hit
GGCTGGAGCTGACCACGTGGTGGAAAGAGCCAGTTTGGGAACTAGGTGTC4130.11189409887320204No Hit
ATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGTTTGTG4110.11135223883023254No Hit
GGCTGGGAGAATGCTGGCTGTACGGAAAGGCTGGAGCTGACCACGTGGTG3750.10159875805678151No Hit
CCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCG3740.10132782803529677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG951.9172585E-645.7905431
GTTACGC657.329699E-444.616433
GTTATAG657.329699E-444.616433
TACTGGG8900.039.1020363
TTACGCA750.001482002138.6675724
ACTGGGA9500.037.3956074
TATTACA9950.036.4330142
ATTACAT9200.036.2508473
GTATTAC9150.035.6565741
GATACTG10000.034.800811
GCGAAAG2103.765308E-1034.5246161
ATACTGG10100.034.456252
TTACATC9150.034.0718354
TGTTACG1102.432852E-432.955322
TAACGAC5000.031.9007433
CGAAAGC2351.2569217E-930.8517862
CTTAACG5200.030.6737941
GTGTAGA2859.094947E-1230.5270291
ACGACTG5300.030.0950435
CTGGGAA12050.030.083695
Produced by FastQC (version 0.11.5)