FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R2_A05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R2_A05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136349
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG17060.1501299336735457No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA16150.14212183052917723No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT12400.10912140548370262No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC12120.10665737374697386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3550.059.24561
CGAAAGC5300.042.4070853
GCGAAAG6600.035.15272
GTATATA20250.027.219181
GTATTAC10100.026.5684851
TACGCCC2202.22857E-523.0689584
TATATGT10250.022.634914
CATACAT11000.022.4078758
TATAGAC13500.022.019262
TAACGAC6100.021.3941653
TTACATC11950.021.2350254
TATACAG12300.021.2202285
TACATAC12450.020.3822126
TCGTTAC2505.9738668E-520.3006842
CTAGGCT7150.020.2804054
TATACCA4652.055458E-1020.2694995
GTAGACG2155.055569E-420.233243
ATATATG9900.019.7733943
CTACGCC2205.902704E-419.7733923
TATTACA14850.019.5292782
Produced by FastQC (version 0.11.5)