FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGCACTAGGTGTG31.9736842105263157No Hit
CCACCATCACGTGCTGGCAACATAGAACGAGGGTTGCGCTCGTTGCGGGA31.9736842105263157No Hit
CTCCTATGAGTCCCCACCATCACGTGCTGGCAACATAGAACGAGGGTTGC31.9736842105263157No Hit
TTGCTGATCTGCGATTACTAGCGACTCCGACTTCACGTGGTCGAGTTGCA31.9736842105263157No Hit
AGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCGTTGCTGATCTGCG21.3157894736842104No Hit
GCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTAC21.3157894736842104No Hit
ATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCCGACTTCACG21.3157894736842104No Hit
AGGTGTGGGGGACATTCCACGTTTTCCGCGCCGTAGCTAACGCATTAAGT21.3157894736842104No Hit
GGCTTGACATACACCGGATCGGCTCAGAGATGGGTTTTCCTCCTTGTGGG21.3157894736842104No Hit
GCTCAGAGATGGGTTTTCCTCCTTGTGGGGCTGGTGTACAGGTGGTGCAT21.3157894736842104No Hit
CGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCCGACTTC21.3157894736842104No Hit
ACTAGCGACTCCGACTTCACGTGGTCGAGTTGCAGACCACGATCCGAACT21.3157894736842104No Hit
GTGTACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGG21.3157894736842104No Hit
GAAACAAGGCCCGGGAACGTATTCACCGCAGCGTTGCTGATCTGCGATTA21.3157894736842104No Hit
CCTGTACACCAGCCCCACAAGGAGGAAAACCCATCTCTGAGCCGATCCGG21.3157894736842104No Hit
GAACCTTACCAAGGCTTGACATACACCGGATCGGCTCAGAGATGGGTTTT21.3157894736842104No Hit
CGATCCGAACTGAGACCAGCTTTTTGGGATTAGCTCCACCTCACGGTATC21.3157894736842104No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG10.6578947368421052No Hit
GCCCAAGACATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCC10.6578947368421052No Hit
AAAGCATGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAA10.6578947368421052No Hit
TGCTCCAGGAGCAGCTGGGGCAAATTTATAGCAGCAAGACGCCGGTATTA10.6578947368421052No Hit
AGACATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGAGTT10.6578947368421052No Hit
TGACATACACCGGATCGGCTCAGAGATGGGTTTTCCTCCTTGTGGGGCTG10.6578947368421052No Hit
GGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTC10.6578947368421052No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA10.6578947368421052No Hit
GTGAAAATACCATGGCCACAATTATTGCTTCGGCAATCGACTGTTCGTCT10.6578947368421052No Hit
GTATCAGCAGATCCACTAGCATCTTTATTGGGGATGTGCCACAGCACATA10.6578947368421052No Hit
ATCACGTGCTGGCAACATAGAACGAGGGTTGCGCTCGTTGCGGGACTTAA10.6578947368421052No Hit
GTTCATTGATACTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA10.6578947368421052No Hit
CTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGT10.6578947368421052No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG10.6578947368421052No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC10.6578947368421052No Hit
TCAATAGGGCGAATCAGCGATTGAATCCTATGCATCAACTTTTAAGACAT10.6578947368421052No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG10.6578947368421052No Hit
GCGTGAAGCCCAAGACATAAGGGGCATGATGATTTGACGTCATCCCCACC10.6578947368421052No Hit
TCCTATGAGTCCCCACCATCACGTGCTGGCAACATAGAACGAGGGTTGCG10.6578947368421052No Hit
TCTTTGCAGTCATCAAAGTCTACTTTTTCTGCTGCCATATTTTAACCGAG10.6578947368421052No Hit
CTCATAGGAGACTGCCGGGGTCAACTCGGAGGAAGGTGGGGATGACGTCA10.6578947368421052No Hit
GAGAAGAAAGAAGAACTCCAGGATTGCAAAATTTCTCCTTTGATGGTTGC10.6578947368421052No Hit
GAGTAAGTTATTGTCAGTTTCTCTGTTCCCTGTGTTTCACTGACCTCCTC10.6578947368421052No Hit
TTATTGTGCTGTCTCATCATTTTGGCAAAGAATTCGAGCTCGGTACCCGG10.6578947368421052No Hit
AAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTT10.6578947368421052No Hit
GGATTCAATCGCTGATTCGCCCTATTGACGAAATTCAGATCACCTCTGAC10.6578947368421052No Hit
GAGATGGGTTTTCCTCCTTGTGGGGCTGGTGTACAGGTGGTGCATGGTTG10.6578947368421052No Hit
GAGCATGCGGATTAATTCGATGCAACGCGAAGAACCTTACCAAGGCTTGA10.6578947368421052No Hit
GACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCA10.6578947368421052No Hit
TCTCTGAGCCGATCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTT10.6578947368421052No Hit
CGCGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGCTCCTTGAGAG10.6578947368421052No Hit
CTTCAAAAGGCACGCTGTCACCCCAAAAGGCTCCAACGGCTTGTAAGCAC10.6578947368421052No Hit
GCTTAGAAGCAATCCCTCCCCACCTGAGCCTCTGATGAGACGACAGCCCT10.6578947368421052No Hit
CGTGATCACCGGCAATGCCGGGCGCAACACGCTGGATGGCGGCGCGGGCA10.6578947368421052No Hit
CCATGATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGATCAGGA10.6578947368421052No Hit
ATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTATGAGTCCCCAC10.6578947368421052No Hit
AACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCCGACT10.6578947368421052No Hit
ATAACACACTCGGTTAAAGACAGATGAAGAAGAGGACCAAAAATAACGAA10.6578947368421052No Hit
GTGTGGGGGACATTCCACGTTTTCCGCGCCGTAGCTAACGCATTAAGTGC10.6578947368421052No Hit
AGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTC10.6578947368421052No Hit
GGTTCCACTTGCGAGGTTGTCCCAAAGTGCATCAAAAATGAGATGCAAGG10.6578947368421052No Hit
CCAAATACTCATTCTGATGTTTTAAATGATTTGCCCTCCCATATGTCCTT10.6578947368421052No Hit
AAAGCAGGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAA10.6578947368421052No Hit
CAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTACACCA10.6578947368421052No Hit
CGCATTAAGTGCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAA10.6578947368421052No Hit
TGCTCATTTCTCTCAGGAATCATTTCCGTTATCCTCTTGTCTGCTGTAAT10.6578947368421052No Hit
AGGAGAGCCTGAAATCCAAACCAGGGCTCCCAGAAAACCACCAACATTGT10.6578947368421052No Hit
TTCCGCGCCGTAGCTAACGCATTAAGTGCCCCGCCTGGGGAGTACGGCCG10.6578947368421052No Hit
ATTCATTTTACTCCATAAAGTTTGTCCTTGCTCATTTCTCTCAGGAATCA10.6578947368421052No Hit
GATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGCACTAGGTGTGG10.6578947368421052No Hit
TTCTCTCTCCTCTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAAT10.6578947368421052No Hit
GATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCTAAATCCCTTAAC10.6578947368421052No Hit
ATGTTGCCAGCACGTGATGGTGGGGACTCATAGGAGACTGCCGGGGTCAA10.6578947368421052No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA10.6578947368421052No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT10.6578947368421052No Hit
CTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCG10.6578947368421052No Hit
GTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAG10.6578947368421052No Hit
GTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGC10.6578947368421052No Hit
TCCGAACTGAGACCAGCTTTTTGGGATTAGCTCCACCTCACGGTATCGCA10.6578947368421052No Hit
AGGTGTGGGGGACATTCCACGATTTCCGCGCCGTAGCTAACGCATAAAGT10.6578947368421052No Hit
GTGAATGTGAGGGGATCAGGAATGAGAATACTTGTAAGGGGCAATTCTCC10.6578947368421052No Hit
CCCTCGTTCTATGTTGCCAGCACGTGATGGTGGGGACTCGTAGGAGACTG10.6578947368421052No Hit
TCTCACGACACGAGCTGACGACAACCATGCACCACCTGTACACCAGCCCC10.6578947368421052No Hit
CATTAAGTGCCCCGCCTGGGGAGTACGGCCGAAAGGCTAAAACTCAAAGG10.6578947368421052No Hit
TCCTTGAGTCAAATGCAACACTTCAATGTACACACTGCTTGTTCCACCAG10.6578947368421052No Hit
CTGGTAGTCCATGCCGTAAACGTTGGGCACTAGGTGTGGGGGACATTCCA10.6578947368421052No Hit
AGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCAC10.6578947368421052No Hit
TATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGA10.6578947368421052No Hit
GGTTAAGTCCCGCAACGAGCGCAACCCTCGTTCTATGTTGCCAGCACGTG10.6578947368421052No Hit
GATCAACATCATCATTCCTCACTTCCCCTCCTGGAGTATACATCTGTTCC10.6578947368421052No Hit
GGTCGTAGCCGTGCCCAGCCACAAGTCACCTAGGGCCCTACACATGGTAA10.6578947368421052No Hit
GAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACA10.6578947368421052No Hit
GGTGTACAGGTGGTGCATGGTAGTCGTCAGCTCGTGTCGTGAGATGTTGG10.6578947368421052No Hit
AGCATGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAAC10.6578947368421052No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG10.6578947368421052No Hit
GGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGCACTGGGTGTG10.6578947368421052No Hit
TAGCTCCACCTCACGGTATCGCAACCCATTGTACTGGCCATTGTAGCATG10.6578947368421052No Hit
CCGTAAACGTTGGGCACTAGGTGTGGGGGACATTCCACGTTTTCCGCGCC10.6578947368421052No Hit
GGCCACTAGGAGTCTATGGGATTCCTTTCGTCAGACCGATTGAGGCGATC10.6578947368421052No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT10.6578947368421052No Hit
GAAATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAG10.6578947368421052No Hit
GACATAAGGGGCATGATGATTTGACGTCATCCCCACCTTACTCCGAGTTG10.6578947368421052No Hit
CCACCTGTACACCAGCCCCACAAGGAGGAAAACCCATCTCTGAGCCGATC10.6578947368421052No Hit
ACCCATCTCTGAGCCGATCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC10.6578947368421052No Hit
AATTCAAACAGCTGCCCAGAGGGGAATGAAGGATGAAGTAAGAGAAAGTC10.6578947368421052No Hit
GAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAA10.6578947368421052No Hit
AGATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGG10.6578947368421052No Hit
GCTCATACCCTCAACGTCTTGCGTCACCCGCAGACTTCCTGGCTATCGCG10.6578947368421052No Hit
CGGCAGTCTCCTATGAGTCCCCACCATCACGTGCTGGCAACATAGAACGA10.6578947368421052No Hit
ACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACA10.6578947368421052No Hit
CCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGGTCC10.6578947368421052No Hit
CCTCCGAGTTGACCCCGGCAGTCTCCTATGAGTCCCCACCATCACGTGCT10.6578947368421052No Hit
GAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA10.6578947368421052No Hit
ACATACACCGGATCGGCTCAGAGATGGGTTTTCCTCCTTGTGGGGCTGGT10.6578947368421052No Hit
TACCAAGGCTTGACATACACCGGATCGGCTCAGAGATGGGTTTTCCTCCT10.6578947368421052No Hit
CCCCTTGAGCATCTGACTTCTGGCTAATAAAGGAAATTTATTTTCATTGC10.6578947368421052No Hit
TGGTAGTCCATGCCGTAAACGTTGGGCACTAGGTGTGGGGGACATTCCAC10.6578947368421052No Hit
TGTCTTGGGCTTCACGCATGCTACAATGGCCAGTACAATGGGTTGCGATA10.6578947368421052No Hit
GTACTGAGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATAG10.6578947368421052No Hit
CACCGGATCGGCTCAGAGATGGGTTTTCCTCCTTGTGGGGCTGGTGTACA10.6578947368421052No Hit
GAGTTGCAGACCACGATCCGAACTGAGACCAGCTTTTTGGGATTAGCTCC10.6578947368421052No Hit
CCATTGTACTGGCCATTGTAGCATGCGTGAAGCCCAAGACATAAGGGGCA10.6578947368421052No Hit
ATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATGCTCAAG10.6578947368421052No Hit
AAATGATCAAGATAATCAGGCATGCCTTACATTTGCACTGTAAGGAAATG10.6578947368421052No Hit
CCGCTTAACAATATTGTTCTTCCAATCAATGAGAATGGATTTGCTTCCCG10.6578947368421052No Hit
GATGGGTTTTCCTCCTTGTGGGGCTGGTGTACAGGTGGTGCATGGTTGTC10.6578947368421052No Hit
CCCAAAGTGAGGGATCGAGAAGGGAGAATGAACTATTACTGGACACTAGT10.6578947368421052No Hit
CAGTTACCAACGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTAT10.6578947368421052No Hit
GAGTGATGGTATCACCTCTGGCTGTGACATGGTGGAATAGGAATGGACCA10.6578947368421052No Hit
ACCAAGGCTTGACATACACCGGATCGGCTCAGAGATGGGTTTTCCTCCTT10.6578947368421052No Hit
CCTGTAGAATCTGTCCACTCCTGCTTGTATTCACTCATGGTTTGGTGCAT10.6578947368421052No Hit
GCTCATACACTCAACGTCTTGCGTCACCCGCAGACTTCCTGGCTATCGCG10.6578947368421052No Hit
GTGGGGGACATTCCACGTTTTCCGCGCCGTAGCTAACGCATTAAGTGCCC10.6578947368421052No Hit
CTCATAGGAGACTGCCGGGGTCAACTCGGAGGAGGTGGGGATGACGTCAA10.6578947368421052No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers