FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650513
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAGCTAAAGTGCCAGGAGAAATGACTAGGAAGGTGGGAAACCCTC16050.2467283513165763No Hit
TTCTTATACATGCCCTTAAGGACCATCTGAATGTGAAGACTCGAGAATTA6780.10422543438793691No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGA100.007089567145.004996
CGAAAGC2150.074.18863
TAACCGG300.001935172572.50254
TATAGAC1200.066.465742
GACGTAT954.5474735E-1061.059425145
CGTATAC805.8690966E-754.3768733
GTTCGGG1004.6844434E-850.7712671
TCCGTAT752.543285E-548.3535841
CTATACT604.9429585E-448.3354
ATTACGC1453.8380676E-1045.0015536
ATATAGC6400.041.9155043
GTATATA6450.041.606571
ACGCACC1651.364242E-939.5437779
TACGCAC1701.8280844E-938.3807268
AGACTAG951.02828606E-438.1592066
ATTCGGT951.02828606E-438.1592066
CATATAG1001.3874909E-436.265191
CCGTATA1209.551419E-636.2540362
TATACTA1001.3906325E-436.251255
TATAGCT7500.034.8011974
Produced by FastQC (version 0.11.5)