FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_H08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_H08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162682
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT20300.17459632126411176No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA17300.14879390925463712No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC16280.14002108917141573No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG15440.13279641380876284No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA13740.11817504700339387No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT13670.1175729907231728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3300.059.343371
CAATTCG3150.046.0295079
GCGAAAG5150.038.0127642
CGAAAGC5600.034.9536553
GTATTAC13250.033.938931
GTTACGC3750.032.865073
CAGCTCG8750.032.3127149
TATACAG9350.031.7898985
GCGTAAC2807.2759576E-1231.0846211
TATTACA15150.030.150942
TATAGAC18400.029.5540052
TTACGCA3950.029.365664
ATAGACC8400.028.4807593
GTATAAT3102.7284841E-1128.0764331
ATTACAT15500.027.5954323
ACATCAA15300.027.4823256
TGGACCG1855.819402E-627.4310975
TTACATC15700.027.2438964
GGACCGT2507.836752E-826.0987326
TACGCAG4450.026.0661495
Produced by FastQC (version 0.11.5)