FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785667
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10340.13160792040393704No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10040.12778950878680154No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA8420.10717008605426982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2300.059.910791
TATACGT805.8743717E-754.371924
CAATTCG3800.043.8790979
CGAAAGC4250.037.5272872
GCGAAAG4600.034.6851961
GATATAG1301.6549731E-533.4596443
AGTAGAA5800.032.5105741
CGTGCTA1601.877268E-631.7169538
GTAGAAA9400.030.8611011
GACAGTA4000.030.8107577
TATATTC5650.029.5116043
GTATACG1555.501065E-528.0629273
GTCGCTC1050.007709591627.6174859
TAGAAAC10050.025.9686782
TAGTACC1701.0314137E-425.5867884
AATATAT7200.025.18181
GTATAGG2051.2865576E-524.7641531
TTATACT5050.024.404564
ATAGACC5700.024.1652983
ATATTCA6300.024.1652984
Produced by FastQC (version 0.11.5)