FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_G07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_G07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234540
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG21860.17707000178204027No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA15980.12944092536491325No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT15320.12409480454258268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4350.060.0305861
CGAAAGC5900.043.0112273
ATAGACG907.48064E-540.2803543
GCGAAAG6250.039.444122
TATACAG11100.032.0026635
GTATATA19950.029.4510961
GTATAGG5100.028.4458681
CAGCTCG10950.027.8066189
TATATAC24700.026.4186953
ATATACT13000.026.2132154
TATATGT11400.026.076234
ATATATG11050.025.5898723
TATACTG5500.025.0440145
TACATAC12450.025.038726
GCACCGT6050.023.9655636
CATACAT12850.023.6951338
ACTATAC3702.6011548E-1023.5150173
GTATTAT6050.022.7802031
TATACTT15300.022.743795
ATACAGC15100.022.5649266
Produced by FastQC (version 0.11.5)